Size and Substitution Effect on Antimicrobial Activity of Polythiophene Polyelectrolyte Derivatives Under Photolysis and Dark Conditions
| Autor: | Edward W. Strach, Dylan M. Brown, Jianzhong Yang, David G. Whitten, Mohammed I Khalil |
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| Rok vydání: | 2018 |
| Předmět: |
Light
Bacillus Microbial Sensitivity Tests Thiophenes 02 engineering and technology 010402 general chemistry medicine.disease_cause Photochemistry 01 natural sciences Biochemistry Polymerization Structure-Activity Relationship Cations Escherichia coli medicine Structure–activity relationship Amines Physical and Theoretical Chemistry chemistry.chemical_classification Reactive oxygen species Microbial Viability Photolysis Photosensitizing Agents biology Photodissociation Imidazoles Cationic polymerization General Medicine 021001 nanoscience & nanotechnology biology.organism_classification Antimicrobial Polyelectrolyte Anti-Bacterial Agents 0104 chemical sciences chemistry Bacillus atrophaeus Reactive Oxygen Species 0210 nano-technology |
| Zdroj: | Photochemistry and Photobiology. 94:1116-1123 |
| ISSN: | 1751-1097 0031-8655 |
| DOI: | 10.1111/php.13013 |
| Popis: | Cationic polythiophenes have been shown to be potent antimicrobial compounds due to their ability to absorb visible light and sensitize the production of reactive oxygen species (ROS) as well as their ability to selectively associate with and damage negatively charged cell envelopes. This study demonstrates the ability of differentially sized imidazolium- and tertiary amine-functionalized poly(3-hexylthiophene) (P3HT) to inactivate Gram-negative Escherichia coli and Gram-positive Bacillus atrophaeus under photolysis and dark conditions. Flow cytometry viability assays are used to quantify cell death. Each compound shows high levels of killing at both 1 and 10 μg mL-1 polymer concentrations for each microbial species after photoactivation as well as high levels of dark inactivation in many cases. Tertiary amine-functionalized P3HT is shown to have different killing patterns, shown by transmission electron microscopy, compared to the imidazolium-functionalized derivatives. |
| Databáze: | OpenAIRE |
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