Self-Rolling Refillable Tubular Enzyme Containers Made of Recombinant Spider Silk and Chitosan
| Autor: | Thomas Scheibel, Tamara B. Aigner |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Materials science
Silk Biocompatible Materials 02 engineering and technology engineering.material 010402 general chemistry 01 natural sciences Enzyme catalysis Chitosan Glucose Oxidase chemistry.chemical_compound Animals General Materials Science Spider silk Semipermeable membrane Horseradish Peroxidase chemistry.chemical_classification Aqueous solution Bilayer Temperature Spiders Hydrogen-Ion Concentration Enzymes Immobilized Fluoresceins 021001 nanoscience & nanotechnology Recombinant Proteins 0104 chemical sciences Enzyme chemistry Chemical engineering Biocatalysis engineering Biopolymer Fibroins 0210 nano-technology Peptide Hydrolases |
| Zdroj: | ACS Applied Materials & Interfaces. 11:15290-15297 |
| ISSN: | 1944-8252 1944-8244 |
| DOI: | 10.1021/acsami.9b01654 |
| Popis: | Encapsulation of enzymes is often necessary to stabilize them against environmental conditions or to protect them from other harmful enzymes such as proteases. Here, a refillable spatial confinement system was produced using a fully degradable self-rolling biopolymer bilayer. The enzyme containers comprise spider silk and chitosan and enable one-pot reactions in the micro- to milliliter regime by trapping the enzyme inside the semipermeable tube and allow the substrate and/or product either to diffuse freely or to be entrapped. The tubes are stable toward several organic and aqueous solvents. A one-tube system with esterase-2 was used to establish the system. Further, a two-tube system was applied to mimic enzymatic cascades, where the enzymes have to be separated, because they, for example, inhibit each other. The entrapment mode was also tested in the two-tube system, which is beneficial for toxic products or for obtaining high concentrations of the desired product. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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