Supercomplex-associated Cox26 protein binds to cytochrome c oxidase
| Autor: | Rosemary A. Stuart, Juliana Heidler, Heike Angerer, Valentina Strecker, Cristina-Maria Cruciat, Kathy Pfeiffer, Zibirnisa Kadeer, Heiko Giese, Ilka Wittig |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Electrophoresis Models Molecular Protein Denaturation Saccharomyces cerevisiae Proteins Cytochrome Protein subunit Population Plasma protein binding Saccharomyces cerevisiae Mitochondrion Catalysis Article Electron Transport Complex IV 03 medical and health sciences Oxygen Consumption Enzyme Stability Cytochrome c oxidase Disulfides education Molecular Biology education.field_of_study 030102 biochemistry & molecular biology biology Wild type Temperature Cell Biology Mitochondria Molecular Weight Protein Subunits 030104 developmental biology Biochemistry Mitochondrial Membranes biology.protein Hydrophobic and Hydrophilic Interactions Protein Binding |
| Zdroj: | Biochimica et biophysica acta. 1863(7 Pt) |
| ISSN: | 0006-3002 |
| Popis: | Here we identified a hydrophobic 6.4 kDa protein, Cox26, as a novel component of yeast mitochondrial supercomplex comprising respiratory complexes III and IV. Multi-dimensional native and denaturing electrophoretic techniques were used to identify proteins interacting with Cox26. The majority of the Cox26 protein was found non-covalently bound to the complex IV moiety of the III–IV supercomplexes. A population of Cox26 was observed to exist in a disulfide bond partnership with the Cox2 subunit of complex IV. No pronounced growth phenotype for Cox26 deficiency was observed, indicating that Cox26 may not play a critical role in the COX enzymology, and we speculate that Cox26 may serve to regulate or support the Cox2 protein. Respiratory supercomplexes are assembled in the absence of the Cox26 protein, however their pattern slightly differs to the wild type III–IV supercomplex appearance. The catalytic activities of complexes III and IV were observed to be normal and respiration was comparable to wild type as long as cells were cultivated under normal growth conditions. Stress conditions, such as elevated temperatures resulted in mild decrease of respiration in non-fermentative media when the Cox26 protein was absent. |
| Databáze: | OpenAIRE |
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