Effects of antisense oligodeoxynucleotides against opioid receptors on the receptor mRNA contents
Autor: | Kayoko Iwao, Hiroyuki Kobayashi, Tomomi Kobayashi, Masanobu Yoshikawa, Tetsuo Oka, Ken Kitamura |
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Rok vydání: | 2001 |
Předmět: |
Male
medicine.medical_specialty Microinjections Receptors Opioid mu Pharmacology Periaqueductal gray General Biochemistry Genetics and Molecular Biology Receptors Opioid delta Receptor mrna Internal medicine polycyclic compounds medicine Animals Periaqueductal Gray RNA Messenger Rats Wistar General Pharmacology Toxicology and Pharmaceutics Receptor Microinjection Messenger RNA Antisense oligodeoxynucleotides Reverse Transcriptase Polymerase Chain Reaction Chemistry Receptors Opioid kappa hemic and immune systems General Medicine Oligonucleotides Antisense respiratory system Rat brain Rats Endocrinology nervous system Opioid human activities medicine.drug |
Zdroj: | Life Sciences. 68:2221-2225 |
ISSN: | 0024-3205 |
Popis: | It was demonstrated in the previous study that the microinjection of antisense oligodeoxynucleotide (AS ODN) against μ-opioid receptor (MOR) into periaqueductal gray (PAG) of rat brain selectively decreased the MOR mRNA content in PAG, and the decrease in MOR mRNA content was enhanced by pretreatment of the PAG with MOR AS ODN. In the present investigation, effects of the pretreatment of PAG with AS ODN against κ- or δ-opioid receptor (KOR or DOR) on the decrease in the MOR mRNA content induced by MOR AS ODN were examined. Both KOR and DOR AS ODNs significantly decreased the target mRNA contents, while they did not significantly change MOR mRNA content. The decrease in MOR mRNA content induced by MOR AS ODN, however, was significantly enhanced by the pretreatment of PAG with either KOR or DOR AS ODNs. Results show that the AS ODN has both the specific target mRNA decreasing action and the nonspecific enhancing action on the AS-induced decrease in the mRNA content. |
Databáze: | OpenAIRE |
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