Presence of 2-hydroxymyristate on endotoxins is associated with death in neonates with Enterobacter cloacae complex septic shock
| Autor: | Simon Barreault, Nada Semaan, Florence Doucet-Populaire, Richard Chaby, Enrique Hernandez Alonso, Pierre Tissieres, Stuti Gera, Véronique Faraut-Derouin, Aude Breton, Daniele De Luca, Nadège Bourgeois-Nicolaos, Luis A. Augusto |
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| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | iScience, Vol 24, Iss 8, Pp 102916-(2021) iScience |
| ISSN: | 2589-0042 |
| DOI: | 10.1016/j.isci.2021.102916 |
| Popis: | Summary Enterobacter cloacae complex species are involved in infections among critically ill patients. After a recent E.cloacae outbreak of fulminant neonatal septic shock, we conducted a study to determine whether septic shock severity and its lethal consequence are related to structural features of the endotoxin (lipopolysaccharide [LPS]) of the strains isolated from hospitalized infants and more specifically its lipid A region. It appeared that the LPSs are very heterogeneous, carrying fifteen different molecular species of lipid A. The virulence was correlated with a structural feature identified by matrix-assisted laser desorption ionization–time of flight mass spectrometry and gas chromatography coupled with mass spectrometry: the presence of 2-hydroxymyristic acid as a secondary substituent in lipid A. This is the first published evidence linking LPS structural moiety to neonatal sepsis outcome and opens the possibility of using this fatty acid marker as a detection tool for high-risk patients, which could help reduce their mortality. Graphical abstract Highlights • Fifteen different molecular species of lipid A is found in E. cloacae complex • 2-Hydroxymyristate moiety on Lipid A is a virulence marker of the E. cloacae complex • Presence of 2-hydroxymyristate is associated with mortality in neonatal sepsis Molecular biology; Immunology; Microbiology |
| Databáze: | OpenAIRE |
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