OsmiR528 regulates rice-pollen intine formation by targeting an uclacyanin to influence flavonoid metabolism
Autor: | Yu-Chan Zhang, Yan-Fei Zhou, Rui-Rui He, Yang Yu, Quan-Feng Li, Jian-Ping Lian, Yu-Wei Yang, Huang He, Meng-Qi Lei, Zhi Zhang, Fan Zhang, Yan-Zhao Feng, Yue-Qin Chen |
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Rok vydání: | 2019 |
Předmět: |
Mutant
Plant Biology Oligopeptide transport Biology medicine.disease_cause uclacyanin intine Microscopy Electron Transmission Gene Expression Regulation Plant Pollen Metalloproteins otorhinolaryngologic diseases medicine Gene miRNA Plant Proteins Multidisciplinary Oryza sativa rice food and beverages Oryza Transporter Biological Sciences Plants Genetically Modified Cell biology MicroRNAs Germination pollen Pollen wall |
Zdroj: | Proceedings of the National Academy of Sciences of the United States of America |
ISSN: | 1091-6490 0027-8424 |
DOI: | 10.1073/pnas.1810968117 |
Popis: | Significance The intine layer of pollen is essential for pollen grain maturation and pollen tube germination. Abnormal intine development causes pollen sterility and affects seed-setting; therefore, the identification of regulators of intine formation is important for elucidating the mechanisms of pollen formation and function, especially for crop breeding. Here, we report a microRNA, OsmiR528, which regulates pollen intine formation and male fertility in rice (Oryza sativa). OsmiR528 directly targets the uclacyanin family member OsUCL23 to regulate flavonoid metabolism and pollen intine development. This study revealed the function of OsmiR528 and an uclacyanin in pollen development. The intine, the inner layer of the pollen wall, is essential for the normal development and germination of pollen. However, the composition and developmental regulation of the intine in rice (Oryza sativa) remain largely unknown. Here, we identify a microRNA, OsmiR528, which regulates the formation of the pollen intine and thus male fertility in rice. The mir528 knockout mutant aborted pollen development at the late binucleate pollen stage, significantly decreasing the seed-setting rate. We further demonstrated that OsmiR528 affects pollen development by directly targeting the uclacyanin gene OsUCL23 (encoding a member of the plant-specific blue copper protein family of phytocyanins) and regulating intine deposition. OsUCL23 overexpression phenocopied the mir528 mutant. The OsUCL23 protein localized in the prevacuolar compartments (PVCs) and multivesicular bodies (MVBs). We further revealed that OsUCL23 interacts with a member of the proton-dependent oligopeptide transport (POT) family of transporters to regulate various metabolic components, especially flavonoids. We propose a model in which OsmiR528 regulates pollen intine formation by directly targeting OsUCL23 and in which OsUCL23 interacts with the POT protein on the PVCs and MVBs to regulate the production of metabolites during pollen development. The study thus reveals the functions of OsmiR528 and an uclacyanin during pollen development. |
Databáze: | OpenAIRE |
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