COMPARISON OF THE ISOELECTRIC AND MOLECULAR WEIGHT PROPERTIES OF TUBULIN SUBUNITS
| Autor: | Ursula Neudeck, Fred Baskin, H. Feit |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Brain Chemistry
Α subunit biology Isoelectric focusing Protein subunit Polyacrylamide Biochemistry Molecular Weight Cellular and Molecular Neuroscience chemistry.chemical_compound Electrophoresis Isoelectric point Tubulin chemistry biology.protein Animals Cattle Electrophoresis Polyacrylamide Gel Isoelectric Focusing Sodium dodecyl sulfate Glycoproteins |
| Zdroj: | Journal of Neurochemistry. 28:697-706 |
| ISSN: | 1471-4159 0022-3042 |
| DOI: | 10.1111/j.1471-4159.1977.tb10616.x |
| Popis: | — Brain tubulin subunits were separated by a combination of isoelectric focusing and electrophoresis in the presence of sodium dodecyl sulfate (SDS) using a two-dimensional polyacrylamide slab gel technique. Isoelectric focusing separated tubulin subunits into two major groups of bands, such that the more acidic group corresponded to the α subunit and the less acidic group corresponded to the β subunit. In addition, isoelectric focusing resolved the β subunit into two subspecies which differed slightly in isoelectric properties but were the same apparent molecular weight. The a subunit was resolved into many subspecies that appear to differ from each other by both apparent molecular weight and isoelectric properties. |
| Databáze: | OpenAIRE |
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