How to grow your cable bacteria: Establishment of a stable single-strain culture in sediment and proposal of Candidatus Electronema aureum GS
| Autor: | Lars Peter Nielsen, Jesper Jensen Bjerg, Caitlin Petro, Casper Thorup, Signe Brokjær, Andreas Bøggild, Tine Sneibjerg Ebsen, Andreas Schramm |
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| Rok vydání: | 2021 |
| Předmět: |
DNA
Bacterial Deltaproteobacteria Geologic Sediments Cultivation Segmented filamentous bacteria Biology Applied Microbiology and Biotechnology Microbiology 03 medical and health sciences RNA Ribosomal 16S Botany Cable bacteria Phylogeny Ecology Evolution Behavior and Systematics 030304 developmental biology Bacteriological Techniques Base Composition 0303 health sciences Genome Strain (chemistry) 030306 microbiology Sediment Sequence Analysis DNA 16S ribosomal RNA biology.organism_classification 6. Clean water Autoclave Freshwater sediment Microbial population biology Candidatus Filament Engineering sciences. Technology Bacteria Desulfobulbaceae |
| Zdroj: | Thorup, C, Petro, C, Bøggild, A, Ebsen, T S, Brokjær, S, Nielsen, L P, Schramm, A & Bjerg, J J 2021, ' How to grow your cable bacteria : Establishment of a stable single-strain culture in sediment and proposal of Candidatus Electronema aureum GS ', Systematic and Applied Microbiology, vol. 44, no. 5, 126236 . https://doi.org/10.1016/j.syapm.2021.126236 Systematic and applied microbiology |
| ISSN: | 0723-2020 |
| DOI: | 10.1016/j.syapm.2021.126236 |
| Popis: | Cable bacteria are multicellular filamentous bacteria within the Desulfobulbaceae that couple the oxidation of sulfide to the reduction of oxygen over centimeter distances via long distance electron transport (LDET). So far, none of the freshwater or marine cable bacteria species have been isolated into pure culture. Here we describe a method for establishing a stable single-strain cable bacterium culture in partially sterilized sediment. By repeated transfers of a single cable bacterium filament from freshwater pond sediment into autoclaved sediment, we obtained strain GS, identified by its 16S rRNA gene as a member of Ca. Electronema. This strain was further propagated by transferring sediment clumps, and has now been stable within its semi-natural microbial community for several years. Its metagenome-assembled genome was 93% complete, had a size of 2.76 Mbp, and a DNA G + C content of 52%. Average Nucleotide Identity (ANI) and Average Amino Acid Identity (AAI) suggest the affiliation of strain GS to Ca. Electronema as a novel species. Cell size, number of outer ridges, and detection of LDET in the GS culture are likewise consistent with Ca. Electronema. Based on these combined features, we therefore describe strain GS as a new cable bacterium species of the candidate genus Electronema, for which we propose the name Candidatus Electronema aureum sp.nov. Although not a pure culture, this stable single-strain culture will be useful for physiological and omics-based studies; similar approaches with single-cell or single-filament transfers into natural medium may also aid the characterization of other difficult-to culture microbes. (c) 2021 The Authors. Published by Elsevier GmbH. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
| Databáze: | OpenAIRE |
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