Transformation of rat embryo cells by adenovirus type 1
| Autor: | McAllister Rm, Lewis Am, Nicolson Mo, Macpherson I, Huebner Rj |
|---|---|
| Rok vydání: | 1969 |
| Předmět: |
Lymphoma
Cell Fluorescent Antibody Technique Biology Virus Adenoviridae chemistry.chemical_compound Antigen Cytopathogenic Effect Viral Virology Culture Techniques medicine Animals Humans Antigens Complement Fixation Tests RNA Embryo Neoplasms Experimental Embryo Mammalian Molecular biology Rats medicine.anatomical_structure Cell Transformation Neoplastic chemistry Cell culture DNA Viral Hybridization Genetic RNA Viral Oncogenic Viruses DNA |
| Zdroj: | The Journal of general virology. 4(1) |
| ISSN: | 0022-1317 |
| Popis: | Summary Primary rat embryo cells were transformed by adenovirus 1. The transformed cells formed foci of multilayered growth in monolayer cultures; cell lines were derived from such foci. A virus stock containing 1.1 × 109 virus particles and 1.5 × 108 p.f.u./ml. contained 2.3 × 102 focus-forming units/ml. when assayed in rat embryo cells. Approximately 4.8 × 106 total virus particles or 6.5 × 105 p.f.u. were required to induce one focus of transformed cells. The transformation rate was 0.0003% for cells exposed to about 2 p.f.u./cell. The transformed cells contained adenovirus 1 specific tumour antigens when tested by immunofluorescent and complement-fixation reactions. They also synthesized adenovirus 1 specific RNA which forms RNase-resistant hybrids with adenovirus 1 DNA. |
| Databáze: | OpenAIRE |
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