Characterization of insulin-like growth factor-binding proteins produced by cultured fibroblasts from patients with noninsulin-dependent diabetes mellitus, insulin-dependent diabetes mellitus, or obesity
| Autor: | S Giannini, Junko Kasuya, S Mohan, G Galli, Y Fujita-Yamaguichi, C M Rotella, T R LeBon |
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| Rok vydání: | 1994 |
| Předmět: |
Adult
Male medicine.medical_specialty Endocrinology Diabetes and Metabolism medicine.medical_treatment Blotting Western Clinical Biochemistry Biochemistry Insulin-like growth factor-binding protein Impaired glucose tolerance Endocrinology In vivo Internal medicine Diabetes mellitus medicine Humans Obesity Cells Cultured Aged biology business.industry Insulin Biochemistry (medical) Fibroblasts Middle Aged medicine.disease Pathophysiology Insulin-Like Growth Factor Binding Proteins Blot Insulin-Like Growth Factor Binding Protein 2 Kinetics Diabetes Mellitus Type 1 Diabetes Mellitus Type 2 Insulin-Like Growth Factor Binding Protein 4 Culture Media Conditioned biology.protein Female Carrier Proteins Insulin-Like Growth Factor Binding Protein 5 business Hormone |
| Zdroj: | The Journal of Clinical Endocrinology & Metabolism. 79:1824-1830 |
| ISSN: | 1945-7197 0021-972X |
| Popis: | To evaluate whether the production of insulin-like growth factor-binding proteins (IGFBPs) is altered in various pathological states due to modification of the hormonal milieu, we analyzed patterns of IGFBPs released into conditioned medium during 48-h serum-free culture of early passages of human skin fibroblasts from control subjects and patients with metabolic disorders. IGFBP-2, -3, -4, and -5 were identified in the conditioned medium by immunoblotting or RIA. Compared with those in eight control subjects by ligand blot analysis, the levels of IGFBP-3, -2, and -5 were reduced to 43%, 47%, and 53% in 10 noninsulin-dependent diabetic patients, respectively, whereas the levels of IGFBP-3 and -2 were reduced to 36% and 23%, respectively, in 3 nondiabetic obese patients with impaired glucose tolerance. In 2 insulin-dependent diabetic patients, the level of IGFBP-3 was reduced by 25% and 40%, respectively, and IGFBP-2 was not detectable. In contrast, a similar level of IGFBP-4 was detected in both normal and patient's conditioned media, except in 1 insulin-dependent diabetic patient. These data indicate that fibroblasts derived from patients with metabolic disorders retain their intrinsic characteristics even after they are removed from their in vivo hormonal milieu. |
| Databáze: | OpenAIRE |
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