DNA in situ hybridization as a diagnostic tool in the discrimination of melanoma and Spitz naevus
| Autor: | H. M. J. Kerstens, P. J. Poddighe, G.N.P. van Muijen, P.E.J. de Wit, Dirk J. Ruiter |
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| Rok vydání: | 1994 |
| Předmět: |
Adult
Male medicine.medical_specialty Pathology Skin Neoplasms Adolescent Aneuploidy In situ hybridization Biology Nodular melanoma Pathology and Forensic Medicine Diagnosis Differential Nevus Epithelioid and Spindle Cell medicine Nevus Humans Child Melanoma In Situ Hybridization Aged Aged 80 and over DNA Middle Aged medicine.disease Dermatology Child Preschool Cutaneous melanoma Histopathology Female Differential diagnosis |
| Zdroj: | The Journal of pathology. 173(3) |
| ISSN: | 0022-3417 |
| Popis: | As the clinical and histological differential diagnosis between Spitz naevus and cutaneous melanoma may be very difficult, we have investigated whether DNA in situ hybridization maybe helpful in resolving this problem. To this end, routinely-processed paraffin sections of 15 typical Spitz naevi, 15 typical nodular melanomas, and five cases originally misdiagnosed as Spitz naevi but which later metastasized and were reclassified as melanoma were analysed using a method previously described (De Wit et al., J Invest Dermatol 1992; 98: 450-458). Microscopical semi-quantitative evaluation revealed that the number of nuclei with supernumerary aberrations of the centromere region of chromosome 1, suggestive of aneuploidy, was significantly different in Spitz naevi and nodular melanoma. The mean number of aberrant nuclei per high power field was 0.41 and 4.01, respectively (P = 0.0001). On applying the results of the typical lesions to the equivocal, originally misdiagnosed lesions, three out of five could be identified as melanoma. These results suggest that the application of DNA in situ hybridization may contribute to the positive identification of histologically equivocal pigmented lesions. The advantages of this technique are that it is cheap, requires little tissue, and can be applied on routinely-processed paraffin sections. |
| Databáze: | OpenAIRE |
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