Interleukin-2 activation of haematopoietic stem cells
| Autor: | Jana Vinklárková, Miroslav Penka, Ladislav Dušek, Martin Klabusay, Michael Doubek, D. Dvořáková, Roman Hájek, Jiří Adler, Ludmila Bourková, Tomas Buchler, Jiří Mayer, E. Janovská, Zdeněk Adam, Zdeněk Kořístek, Jiří Vorlíček |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Interleukin 2
CD34 Bone Marrow Cells Polymerase Chain Reaction Cell therapy 03 medical and health sciences 0302 clinical medicine Bone Marrow Leukemia Myelogenous Chronic BCR-ABL Positive Medicine Lymphocyte Count Progenitor cell Cells Cultured business.industry General Medicine Flow Cytometry Hematopoietic Stem Cells 3. Good health Transplantation Haematopoiesis medicine.anatomical_structure 030220 oncology & carcinogenesis Immunology Interleukin-2 Regression Analysis Bone marrow Stem cell business Multiple Myeloma 030215 immunology medicine.drug |
| Zdroj: | Acta medica Austriaca. 29(2) |
| ISSN: | 0303-8173 |
| Popis: | BACKGROUND: Recent findings concerning the role of immunity in the eradication of residual malignant disease after autologous haematopoietic stem cell transplantation have led to extensive studies of T-cell and natural killer (NK) mediated anti-tumour effects. Interleukin 2 (IL-2) activation of autologous bone marrow (BM) or peripheral blood stem cells (PBSC) before transplantation is one of the methods of adoptive cell therapy. METHODS: Autologous BM of patients with chronic myelogenous leukaemia (n = 11) and PBSC of patients with multiple myeloma (n = 14) were activated by IL-2 in laboratory conditions with the aim of evaluating the feasibility of this method, the activation of T and NK cells, recovery of active progenitor cells, microbial contamination, and reduction of malignant cell content. RESULTS: Samples of BM (mean 2.6 x 10(6) cells) and PBSC (mean 10.3 x 10(6) cells) were cultured in complete culture medium with IL-2 (6000 Ul/ml) for 24 h. The recovery of CD34+ cells and CFU-GM was 82.5% and 51.5%, respectively, for BM, and 85% and 86%, respectively, for PBSC (mean values). No purging effect was detected by flow cytometry and a small decline in malignant cell contamination was observed by quantitative PCR in BM samples. No microbial contamination occurred during the sample processing. CONCLUSIONS: The described in vitro activation of BM and peripheral blood stem cells using IL-2 was evaluated as a safe and reliable method suitable for clinical application. |
| Databáze: | OpenAIRE |
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