Peroxidase profiling reveals genetic linkage between peroxidase gene clusters and basal host and non-host resistance to rusts and mildew in barley

Autor: Zuzana Kohutova, Piet Stam, Thierry C. Marcel, Ana M. González, Rients E. Niks, C. Gerard van der Linden
Přispěvatelé: Wageningen University and Research [Wageningen] (WUR), BIOlogie et GEstion des Risques en agriculture (BIOGER), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Xunta de Galicia AA-123Czech University of Agriculture AA-123
Jazyk: angličtina
Rok vydání: 2010
Předmět:
0106 biological sciences
Genetic Linkage
Plant Biology
DNA sequences
01 natural sciences
Genetics and Genomics/Plant Genetics and Gene Expression
CANDIDATE GENES
Laboratorium voor Plantenveredeling
class-iii peroxidases
2. Zero hunger
Genetics
0303 health sciences
Multidisciplinary
biology
EPS-2
Chromosome Mapping
food and beverages
Peroxidases
Multigene Family
Medicine
Sequence databases
Powdery mildew
Genome
Plant
Peroxidase
Research Article
Plant Biology/Plant-Biotic Interactions
Genetic Markers
Quantitative trait loci
disease resistance
quantitative resistance
QUANTITATIVE TRAIT LOCUS
Science
MOLECULAR MARKERS
GENE FAMILY
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
arabidopsis-thaliana
Quantitative trait locus
Plant disease resistance
diversity
03 medical and health sciences
Plant Biology/Plant Genetics and Gene Expression
Gene mapping
puccinia-hordei
Barley
expression
Gene
DNA sequence analysis
030304 developmental biology
Plant Diseases
Puccinia
Polymorphism
Genetic
Gene Expression Profiling
consensus map
PEROXIDASE
Hordeum
near-isogenic lines
Sequence Analysis
DNA
biology.organism_classification
Immunity
Innate
Plant Breeding
Sequence motif analysis
biology.protein
powdery mildew
Puccinia hordei
010606 plant biology & botany
Zdroj: PLoS ONE
PLoS ONE, Public Library of Science, 2010, 5 (8), pp.1-11. ⟨10.1371/journal.pone.0010495⟩
PLoS ONE, 5(8)
Plos One 8 (5), 1-11. (2010)
PLoS ONE, Vol 5, Iss 8, p e10495 (2010)
PLoS ONE 5 (2010) 8
Digital.CSIC. Repositorio Institucional del CSIC
instname
ISSN: 1932-6203
Popis: Background: Higher plants possess a large multigene family encoding secreted class III peroxidase (Prx) proteins. Peroxidases appear to be associated with plant disease resistance based on observations of induction during disease challenge and the presence or absence of isozymes in resistant vs susceptible varieties. Despite these associations, there is no evidence that allelic variation of peroxidases directly determines levels of disease resistance. Methodology/Principal Findings: The current study introduces a new strategy called Prx-Profiling. We showed that with this strategy a large number of peroxidase genes can be mapped on the barley genome. In order to obtain an estimate of the total number of Prx clusters we followed a re-sampling procedure, which indicated that the barley genome contains about 40 peroxidase gene clusters. We examined the association between the Prxs mapped and the QTLs for resistance of barley to homologous and heterologous rusts, and to the barley powdery mildew fungus. We report that 61% of the QTLs for partial resistance to P. hordei, 61% of the QTLs for resistance to B. graminis and 47% of the QTLs for non-host resistance to other Puccinia species co-localize with Prx based markers. Conclusions/Significance: We conclude that Prx-Profiling was effective in finding the genetic location of Prx genes on the barley genome. The finding that QTLs for basal resistance to rusts and powdery mildew fungi tend to co-locate with Prx clusters provides a base for exploring the functional role of Prx-related genes in determining natural differences in levels of basal resistance. © 2010 González et al.
Databáze: OpenAIRE
Externí odkaz: