The effect of commercial conjugated linoleic acid products on experimental periodontitis and diabetes mellitus in Wistar rats
| Autor: | Hatice Balci Yuce, Nihat Akbulut, Seda Ocakli, Omer Kayir, Mahfuz Elmastas |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Time Factors endocrine system diseases medicine.medical_treatment Conjugated linoleic acid Interleukin-1beta Alveolar Bone Loss Dentistry Nitric Oxide Synthase Type II Osteoclasts Apoptosis medicine.disease_cause chemistry.chemical_compound Random Allocation 0302 clinical medicine Linoleic Acids Conjugated bcl-2-Associated X Protein food and beverages General Medicine Liver medicine.drug medicine.medical_specialty Streptozocin Diabetes Mellitus Experimental 03 medical and health sciences Internal medicine Diabetes mellitus medicine Animals Rats Wistar Ligature Periodontitis General Dentistry Dental alveolus Triglycerides Osteoblasts business.industry Tartrate-Resistant Acid Phosphatase Cholesterol HDL nutritional and metabolic diseases 030206 dentistry Cholesterol LDL Streptozotocin medicine.disease Rats Oxidative Stress 030104 developmental biology Endocrinology chemistry Hyperglycemia business Oxidative stress Gingival margin |
| Zdroj: | Acta odontologica Scandinavica. 75(1) |
| ISSN: | 1502-3850 |
| Popis: | The aim of present study was to determine the effects of conjugated linoleic acid enriched milk on alveolar bone loss, hyperglycaemia, oxidative stress and apoptosis in ligature-induced periodontal disease in diabetic rat model.Wistar rats were divided into six experimental groups: 1; non-ligated (NL, n = 6) group, 2; ligature only (LO, n = 6) group, 3; streptozotocin only (STZ, n = 8) group, 4; STZ and ligature (STZ + L, n = 8) group, 5; ligature and conjugated linoleic acid (CLA) (L + CLA, n = 8) group, 6; STZ, ligature and CLA group (STZ + L + CLA, n = 8) group. Diabetes mellitus was induced by 60 mg/kg streptozotocin. Rats were fed with CLA enriched milk for four weeks. Silk ligatures were placed at the gingival margin of lower first molars of mandibular quadrant. The study duration was four weeks after diabetes induction and the animals were sacrificed at the end of this period. Changes in alveolar bone levels were clinically measured and tissues were histopathologically examined. Inducible nitric oxide synthase (iNOS) and Bax protein expressions, serum interleukin-1β (IL-1β), low-density lipoprotein (LDL), high-density lipoprotein (HDL) and triglyceride levels and tartrate resistant acid phosphatase (TRAP)+ osteoclast numbers were also evaluated.At the end of four weeks, alveolar bone loss was significantly higher in the STZ + LO group compared to the other groups (p .05). CLA decreased alveolar bone loss in L + CLA and STZ + L + CLA groups. CLA significantly decreased TRAP + osteoclast numbers and increased osteoblastic activity compared to the STZ + L group (p .05). Diabetes and CLA increased Bax protein levels (p .05) however CLA had no effect on iNOS expression (p .05).Within the limits of this study, commercial CLA product administration in addition to diet significantly reduced alveolar bone loss, increased osteoblastic activity and decreased osteoclastic activity in the diabetic Wistar rats. |
| Databáze: | OpenAIRE |
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