Novel human testis-specific histone H2B encoded by the interrupted gene on the X chromosome
| Autor: | Kangling Zhang, Maria Svetlova, Dmitri Churikov, Joseph S. Siino, Andrei O. Zalensky, E. Morton Bradbury, Arunas Gineitis |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Male
endocrine system X Chromosome Pan troglodytes Polyadenylation Green Fluorescent Proteins Molecular Sequence Data Electrophoretic Mobility Shift Assay CHO Cells Biology Evolution Molecular Histones Cricetinae Sequence Homology Nucleic Acid Testis Gene expression Genetics Histone H2B Animals Humans Amino Acid Sequence Epigenetics Promoter Regions Genetic Gene Cells Cultured Cell Nucleus Interrupted gene Telomere Spermatozoa Molecular biology Introns Alternative Splicing Histone Gene Expression Regulation Histone methyltransferase biology.protein |
| Zdroj: | Genomics. 84:745-756 |
| ISSN: | 0888-7543 |
| DOI: | 10.1016/j.ygeno.2004.06.001 |
| Popis: | Testis-specific histones are synthesized and accumulated at specific stages of mammalian spermatogenesis. Their proposed functions range from facilitation of the replacement of somatic histones by protamines to epigenetic control of gene transcription. Several testis histone variants were characterized in mouse and rat; however, few are known in humans. Here we report the identification and characterization of a novel human histone 2B gene (TH2B-175) located at Xq22.2, which encodes a highly divergent H2B variant. The TH2B-175 gene contains two introns and is transcribed exclusively in testis, where the spliced polyadenylated mRNA was detected. Genomic PCR, Southern blot analysis, and BLAST-based searches indicate that TH2B-175 evolved in the primate lineage or has been lost in rodents. In transfected Chinese hamster cells, GFP-tagged TH2B-175 targeted to large fluorescent bodies that partially colocalize with the interstitial telomeric blocks. Therefore, TH2B-175 may have telomere-associated functions and participate in the telomere-binding complex in the human sperm [1]. |
| Databáze: | OpenAIRE |
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