Lytic potential of Lysobacter capsici VKM B-2533T: bacteriolytic enzymes and outer membrane vesicles
| Autor: | I. Yu. Toropygin, A. O. Borovikova, N. E. Suzina, Irina V. Kudryakova, Alexey S. Afoshin, N. A. Shishkova, Natalia V. Vasilyeva |
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| Rok vydání: | 2020 |
| Předmět: |
Methicillin-Resistant Staphylococcus aureus
0301 basic medicine Proteases medicine.medical_treatment 030106 microbiology lcsh:Medicine Lysobacter Article Microbiology Applied microbiology 03 medical and health sciences Minimum inhibitory concentration Anti-Infective Agents Bacterial Proteins Endopeptidases medicine lcsh:Science Secretion chemistry.chemical_classification Membranes Multidisciplinary Protease biology Antimicrobials lcsh:R Antimicrobial biology.organism_classification Bacterial Outer Membrane 030104 developmental biology Enzyme chemistry lcsh:Q Bacterial outer membrane Bacteria |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-13 (2020) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-020-67122-2 |
| Popis: | Recent recurrent outbreaks of bacterial resistance to antibiotics have shown the critical need to identify new lytic agents to combat them. The species Lysobacter capsici VKM B-2533T possesses a potent antimicrobial action against a number of bacteria, fungi and yeasts. Its activity can be due to the impact of bacteriolytic enzymes, antibiotics and peptides. This work isolated four homogeneous bacteriolytic enzymes and a mixture of two proteins, which also had a bacteriolytic activity. The isolates included proteins identical to L. enzymogenes α- and β-lytic proteases and lysine-specific protease. The proteases of 26 kDa and 29 kDa and a protein identified as N-acetylglycosaminidase had not been isolated in Lysobacter earlier. The isolated β-lytic protease digested live methicillin-resistant staphylococcal cells with high efficiency (minimal inhibitory concentration, 2.85 μg/mL). This property makes the enzyme deserving special attention. A recombinant β-lytic protease was produced. The antimicrobial potential of the bacterium was contributed to by outer membrane vesicles (OMVs). L. capsici cells were found to form a group of OMVs responsible for antifungal activity. The data are indicative of a significant antimicrobial potential of this bacterium that requires thorough research. |
| Databáze: | OpenAIRE |
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