Virome Sequencing in Patients With Myocarditis
| Autor: | Wolfgang Poller, W. Ian Lipkin, David M. Leistner, Marina Lovrinovic, Alexandra Oleynik, Paul J. Hanson, Komal Jain, Thomas F. Lüscher, Ulf Landmesser, Nishay Mishra, Frank Enseleit, Bettina Heidecker, Thomas Briese, Jan Berg, Jan Kottwitz, Andrea Baltensweiler, Bruce M. McManus, Alexander Lauten, Simon H. Williams, Nora Baltensperger, Dimitri Patriki, Joel A. Garcia |
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| Přispěvatelé: | University of Zurich, Heidecker, Bettina |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Adult
Male Myocarditis Biopsy Endogenous retrovirus 610 Medicine & health 030204 cardiovascular system & hematology Virus 2705 Cardiology and Cardiovascular Medicine law.invention 11459 Center for Molecular Cardiology 03 medical and health sciences 0302 clinical medicine law Humans Medicine In patient Human virome Polymerase chain reaction 030304 developmental biology 0303 health sciences business.industry High-Throughput Nucleotide Sequencing Middle Aged medicine.disease Virology Virus Diseases Giant cell DNA Viral Viruses RNA Viral 570 Life sciences biology Female Cardiology and Cardiovascular Medicine business |
| Popis: | Background: Polymerase chain reaction analyses of cardiac tissues have detected viral sequences in up to 67% of cases of myocarditis. However, viruses have not been implicated in giant cell myocarditis (GCM). Furthermore, efforts to detect viruses implicated in myocarditis have been unsuccessful in more accessible samples such as peripheral blood. Methods: We used Virome Capture Sequencing for Vertbrate Viruses (VirCapSeq-VERT), a method that simultaneously screens for all known vertebrate viruses, to investigate viruses in 33 patients with myocarditis. We investigated peripheral blood mononuclear cells (n=24), plasma (n=27), endomyocardial biopsies (n=2), and cardiac tissue samples from explanted hearts (n=13). Results: Nine patients (27%) had GCM and 4 patients (13%) had fulminant myocarditis. We found the following viruses in the blood of patients with myocarditis: Epstein Barr virus (n=11, 41%), human pegivirus (n=1, 4%), human endogenous retrovirus K (n=27, 100%), and anellovirus (n=15, 56%). All tissue samples from fulminant myocarditis (n=2) and GCM (n=13) contained human endogenous retrovirus K. Conclusions: No nucleic acids from viruses previously implicated in myocarditis or other human illnesses were detected in relevant amounts in cardiac tissue samples from GCM or in blood samples from other types of myocarditis. These findings do not exclude a role for viral infection in GCM but do suggest that if viruses are implicated, the mechanism is likely to be indirect rather than due to cytotoxic infection of myocardium. |
| Databáze: | OpenAIRE |
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