Temporal temperature gradient gel electrophoresis (TTGE) as a tool for identification of Lactobacillus casei, Lactobacillus paracasei, Lactobacillus zeae and Lactobacillus rhamnosus
| Autor: | Siv Ahrné, Göran Molin, Bertil Pettersson, Alejandra Vásquez |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Electrophoresis
Agar Gel Lactobacillus casei biology Lactobacillus paracasei Temperature Genetic Variation food and beverages Lactobacillus zeae Sequence Analysis DNA biology.organism_classification medicine.disease_cause 16S ribosomal RNA Polymerase Chain Reaction Applied Microbiology and Biotechnology Bacterial Typing Techniques Microbiology Lactobacillus RNA Bacterial Species Specificity Lactobacillus rhamnosus RNA Ribosomal 16S medicine Escherichia coli Temperature gradient gel electrophoresis |
| Zdroj: | Letters in Applied Microbiology. 32:215-219 |
| ISSN: | 0266-8254 |
| DOI: | 10.1046/j.1472-765x.2001.00901.x |
| Popis: | Aims: To develop a tool for rapid and inexpensive identification of the Lactobacillus casei complex. Methods and Results:Lactobacillus casei, Lactobacillus paracasei, Lactobacillus zeae and Lactobacillus rhamnosus were identified by PCR-amplification of the segment between the U1 and U2 regions of 16S rDNA (position 8–357, Escherichia coli numbering) and temporal temperature gradient gel electrophoresis (TTGE). Seven tested Lact. paracasei strains were divided into three TTGE-subgroups. Conclusions: TTGE successfully distinguished between the closely-related target species. TTGE is also a powerful method for revealing sequence heterogeneities in the 16S rRNA genes. Significance and Impact of the Study: Due to rapid and easy performance, TTGE of PCR-amplified 16S rDNA fragments will be useful for the identification of extended numbers of isolates. |
| Databáze: | OpenAIRE |
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