Analysis of the Proenkephalin Second Messenger-Inducible Enhancer in Rat Striatal Cultures
| Autor: | Jean-Christophe Leveque, Patrick Senatus, Alexia E. Pollack, Rebecca L. Cole, Sarah Jane Grossbard, Christine Konradi, Donnella S. Green, Steven E. Hyman |
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| Rok vydání: | 2002 |
| Předmět: |
medicine.medical_specialty
Adenosine 4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone Molecular Sequence Data Adenosine-5'-(N-ethylcarboxamide) Biology CREB Second Messenger Systems Biochemistry Rats Sprague-Dawley Cellular and Molecular Neuroscience Fetus Internal medicine Gene expression Cyclic AMP medicine Cyclic AMP Response Element-Binding Protein Animals RNA Messenger Phosphorylation Protein Precursors Enhancer Cells Cultured Base Sequence Binding protein Colforsin Genes fos Enkephalins Corpus Striatum Rats Proenkephalin Cell biology Transcription Factor AP-1 Enhancer Elements Genetic Endocrinology Second messenger system biology.protein Cyclic AMP Response Element |
| Zdroj: | Europe PubMed Central |
| ISSN: | 0022-3042 |
| DOI: | 10.1046/j.1471-4159.1995.65031007.x |
| Popis: | We have previously shown that in cell extracts from rat striatum, cyclic AMP response element (CRE) binding protein (CREB), rather than AP-1 proteins, preferentially interacts with the CRE-2 element of the proenkephalin second messenger-inducible enhancer, even under conditions in which AP-1 proteins are highly induced. Here we use primary striatal cultures to permit a more detailed analysis of CRE-2 function and protein binding in relevant neural cell types. By transfection we find that in primary striatal cultures, as in transformed cell lines, the CRE-1 and CRE-2 elements are required for significant induction by cyclic AMP. We report that cyclic AMP induction of the proenkephalin gene in striatal cultures is protein synthesis independent, excluding a role for newly synthesized proteins like c-Fos. We also show that cyclic AMP induces CREB phosphorylation and that phosphorylated CREB interacts strongly with CRE-2 and weakly with CRE-1. The predominant protein bound to CRE-1 is not CREB, however, and remains to be identified. Despite some prior predictions, we do not find a role for c-Fos in cyclic AMP regulation of proenkephalin gene expression in neurons. |
| Databáze: | OpenAIRE |
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