The Effect of Follicular-fluid Exposure of Endometriosis Cyst Patients toward the Levels of Bcl-2 and Cytochrome-C in Mice Oocyte
| Autor: | Bagus Komang Satriyasa, Ketut Suwiyoga, I Made Jawi, I Nyoman Mantik Astawa, Ida Bagus Putra Manuaba, I Wayan Putu Sutirtayasa, Ida Bagus Putra Adnyana, Mochammad Anwar, I Made Bakta |
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| Rok vydání: | 2020 |
| Předmět: |
Infertility
biology business.industry Cytochrome c Cytochrome C Endometriosis 030209 endocrinology & metabolism General Medicine Endometriosis Cysts medicine.disease Oocyte Follicular fluid Follicular Fluid Andrology 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure Apoptosis Statistical significance biology.protein Medicine Bcl-2 Cyst 030212 general & internal medicine business |
| Zdroj: | Open Access Macedonian Journal of Medical Sciences; Vol. 8 No. A (2020): A-Basic Sciences; 567-570 |
| ISSN: | 1857-9655 |
| DOI: | 10.3889/oamjms.2020.4791 |
| Popis: | BACKGROUND: Endometriosis cysts adversely affect a woman’s quality of life, which causes pain and reduces fertility. The World Health Organization found that the incidence of endometriosis with infertility clinical manifestations is around 10%. AIM: The purpose of this study is to determine the presence of mice oocytes mitochondrial apoptosis exposed with endometriosis cysts follicular fluid through Bcl-2 and cytochrome C analysis. METHODS: This study was a randomized post-test only control group design conducted at Sanglah Hospital, Bali Royal Hospital in Denpasar, and the Medical Faculty Udayana University from June 2018 to April 2019. A total of 120 mice oocytes were distributed randomly into three groups, i.e., treatment Groups 1 and 2 that added by 15% endometriosis cysts follicular fluid and control group. Bcl-2 and Cytochrome C analyzed with ELISA Technique. Comparability was tested with one-way ANOVA with a significance level of p < 0.05. RESULTS: Eighteen mice oocyte replicates were normally distributed and homogeneous. The Bcl-2 levels in the treatment Groups 1, and 2 and control were 627.83 ± 146.42, 634.50 ± 140.62, and 678.83 ± 152.71, respectively; p = 0.838 (not significantly different). Cytochrome C levels in the treatment Groups 1, and 2, and control, respectively, were 3147.75 ± 228.50, 3104.45 ± 211.29, and 2738.28 ± 227.45; p = 0.021 (significantly different). CONCLUSION: The effect of endometriosis cysts follicular fluid exposure on mitochondrial apoptosis is proven through Cytochrome C, whereas Bcl-2 is not proven. |
| Databáze: | OpenAIRE |
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