Convergent pathways for lipochitooligosaccharide and auxin signaling in tobacco cells
| Autor: | Ursula Wieneke, Jurgen G. Schmidt, Horst Röhrig, Jeff Schell, Inge Czaja, Richard Walden, Michael John, Helge Lubenow |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Lipopolysaccharides
Cell signaling Cytokinins Transcription Genetic Cell division Recombinant Fusion Proteins Biology Transfection chemistry.chemical_compound Glycolipid Plant Growth Regulators Gene Expression Regulation Plant Auxin Tobacco Promoter Regions Genetic Gene Cells Cultured Glucuronidase chemistry.chemical_classification Multidisciplinary Dose-Response Relationship Drug Indoleacetic Acids Models Genetic Arabidopsis Proteins Protoplasts fungi food and beverages Drug Synergism Biological Sciences Blotting Northern Plant cell chemistry Biochemistry Cytokinin Signal transduction Cell Division Signal Transduction |
| Zdroj: | Proceedings of the National Academy of Sciences. 93:13389-13392 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | Lipochitooligosaccharides (LCOs) secreted by rhizobia play a key role in the induction of nodules on the roots of legumes by activating signaling events that, at very low concentration, initiate cell division at distinct sites in the root (1, 2). Possibly these signals affect regulation of the plant cell cycle (3), and evidence is accumulating that LCOs play a general role as plant growth regulators (4–6). Previously we have developed methods to synthesize LCOs and demonstrated that these signaling molecules, which trigger nodulation in legumes, also promote cell division in tobacco protoplasts grown in the absence of auxin and cytokinin (7). Notably, glycolipid signals carrying fatty acids with double bonds in the trans configuration were efficient at femtomolar concentration to alleviate the requirement for micromolar concentrations of auxin and cytokinin to sustain growth of cultured tobacco protoplasts. LCOs increased the levels of AXI1 transcripts in protoplasts grown in the absence of auxin. The AXI1 gene is auxin- and cytokinin-responsive and its deregulated expression uncouples protoplasts from the normal effects of auxin on cell division (8). In this study, we have investigated the minimum structural requirements for LCOs to induce cell division of tobacco protoplasts. We have used the AXI1 promoter in transient expression assays to show that multiple signaling pathways for AXI1 expression exist in tobacco cells. |
| Databáze: | OpenAIRE |
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