Controlled oxidation of calf thymus DNA to produce standard samples for 8-Oxodeoxyguanosine analysis; Effects of freeze-drying, storage and hydrolysis conditions
| Autor: | Andrew Collins, Catherine M. Gedik, Sharon G. Wood |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Standard sample
Pyrrolidines Light Thymus Gland Biochemistry Freeze-drying Hydrolysis chemistry.chemical_compound Drug Stability Animals Chromatography High Pressure Liquid Nuclease Photosensitizing Agents Chromatography biology Deoxyguanosine Phosphodiesterase 8-Hydroxy-2'-deoxyguanosine DNA General Medicine Freeze Drying chemistry 8-Hydroxy-2'-Deoxyguanosine biology.protein Alkaline phosphatase Cattle Oxidation-Reduction Quinolizines |
| Zdroj: | Free Radical Research. 32:327-332 |
| ISSN: | 1029-2470 1071-5762 |
| DOI: | 10.1080/10715760000300321 |
| Popis: | Calf thymus DNA containing defined levels of 8-hydroxy-2'-deoxyguanosine (8-oxodG) was prepared by treatment with visible light in the presence of photosensitiser Ro 19-8022. The DNA was checked for stability; after freeze-drying, the amount of 8-oxodG did not increase during 6 weeks' storage at room temperature. However, freeze-drying itself can introduce additional oxidative damage. Two enzymic hydrolysis regimes (DNase I, phosphodiesterases I and II, and alkaline phosphatase; or P1 nuclease and alkaline phosphatase) give similar values for 8-oxodG. |
| Databáze: | OpenAIRE |
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