Inhibition of the human intermediate-conductance, Ca2+-activated K+ channel by intracellular acidification
| Autor: | Nanna K. Jorgensen, Kamilla Angelo Pedersen, Søren-Peter Olesen, Bo Skaaning Jensen |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Intracellular Fluid
Patch-Clamp Techniques Potassium Channels Physiology Intracellular pH Clinical Biochemistry Gating Acid-Base Imbalance Biology Kidney Cell Line Potassium Channels Calcium-Activated Physiology (medical) Potassium Channel Blockers Extracellular Humans Receptor Sensory mechanism Conductance Intracellular acidification Hydrogen-Ion Concentration Intermediate-Conductance Calcium-Activated Potassium Channels Cytosol Biochemistry Potassium Biophysics Extracellular Space Acids Ion Channel Gating |
| Zdroj: | Pflügers Archiv - European Journal of Physiology. 440:153-156 |
| ISSN: | 1432-2013 0031-6768 |
| DOI: | 10.1007/s004240000269 |
| Popis: | The effect of changes in pH on the gating properties of the cloned human intermediate-conductance, Ca2+-activated K+ channel (hIK) was studied using the patch-clamp technique. Multi-channel inside-out recordings of patches from HEK-293 cells stably expressing hIK channels revealed that the channel activity is modulated by changes in intracellular pH (pHi). Changes in extracellular pH (pHo) in the range from pH 6.0 to 8.2 did not affect the hIK whole-cell current. Intracellular acidification gradually decreased the activity of the hIK channel, approaching zero activity at pHi 6.0. Decreasing pHi altered neither the conductance nor the inward rectification of hIK channels. The proton-induced inhibition of the multi-channel hIK patch current could not be counteracted by increasing the cytosolic Ca2+ concentration to 30 microM. The molecular sensory mechanism underlying the proton-induced modulation of hIK gating is at present unknown. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink