LncRNA SNHG1 regulates vascular endothelial cell proliferation and angiogenesis via miR-196a
| Autor: | Liping Zhang, Yutao Wu, Lingxia Lv, Qiang Zhang, Zhu Jianhua, Chen Ting |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Histology Physiology Angiogenesis Proinflammatory cytokine 03 medical and health sciences Downregulation and upregulation Human Umbilical Vein Endothelial Cells Humans Cell Proliferation Mitogen-Activated Protein Kinase 6 Neovascularization Pathologic 030102 biochemistry & molecular biology Tumor Necrosis Factor-alpha Chemistry Cell growth Autophagy Endothelial Cells Vascular endothelial cell proliferation Cell Biology General Medicine Cell biology MicroRNAs 030104 developmental biology Gene Expression Regulation RNA Interference RNA Long Noncoding Developmental biology Function (biology) |
| Zdroj: | Journal of Molecular Histology. 51:117-124 |
| ISSN: | 1567-2387 1567-2379 |
| Popis: | Inflammatory cytokines are important protagonists in the formation of atherosclerotic plaques, triggering effects throughout the atherosclerotic vessels due to the destruction in proliferation, migration and angiogenesis of endothelial cells. In this study, we found SNHG1 is upregulated in TNF-α-treated HUVECs. We silenced SNHG1 and found it inhibited vascular endothelial cell proliferation and angiogenesis. In the other hand, exogenetic overexpression of SNHG1 promotes proliferation, migration and angiogenesis. Then we demonstrated that SNHG1 may interact directly with miR-196a to act as a miR-196a sponge. Further, MAPK6 were predicted to be the target of miR-196a. So we blocked miR-196a, which increased expression level of MAPK6, enhanced cell proliferation, migration and angiogenesis. These data indicated that SNHG1/miR-196a/MAPK6 axis may take a part in autophagy regulation in TNF-α-treated HUVECs. The subsequent rescue experiments come to the results ascertained the specificity of SNHG1/miR-196a/MAPK6 axis in regulating MAPK6. Overall, our findings demonstrate a novel mechanism by which SNHG1 overexpression protects the function of HUVECs, which may delay the progression of AS. SNHG1/miR-196a/MAPK6 axis may be of therapeutic significance in AS. |
| Databáze: | OpenAIRE |
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