Influence of TNF and IL10 gene polymorphisms in the immunopathogenesis of leprosy in the south of Brazil
| Autor: | Danilo Santana Alessio Franceschi, Paulo R. Peixoto, Luiza T. Tsuneto, Ana Maria Sell, Cristiane Conceição Chagas Rudnick, Priscila Saamara Mazini, Jeane Eliete Laguila Visentainer, Maria Lúcia Ribas |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Microbiology (medical) TNF−308 Genotype Tuberculoid leprosy Single-nucleotide polymorphism Biology Polymerase Chain Reaction Polymorphism Single Nucleotide Cytokine polymorphisms Gene Frequency Reference Values Leprosy Immunopathology Ethnicity medicine Humans Genotyping Lepromatous leprosy Polymorphism Genetic Tumor Necrosis Factor-alpha Haplotype General Medicine medicine.disease Interleukin-10 Infectious Diseases Immunology Female Brazil SNPs |
| Zdroj: | International Journal of Infectious Diseases. 13:493-498 |
| ISSN: | 1201-9712 |
| DOI: | 10.1016/j.ijid.2008.08.019 |
| Popis: | Summary Objective To determine whether cytokine polymorphisms are associated with leprosy and/or their subtypes in a Brazilian population. Methods Genotyping using polymerase chain reaction with sequence-specific primers (PCR-SSP) was performed for: TNF −308/−238 , IL2 −330/+166 , IL6 −174 , IFNG +874 , TGFB1 +869/+915 , and IL10 −592/−819/−1082 in 240 healthy controls and 167 patients with leprosy. Results For TNF −308 , a higher frequency of GG genotype (85.5% vs. 74.1% in healthy controls, p =0.009), along with a decreased frequency of GA/AA genotypes was observed among leprosy patients as compared to the control group (14.5% vs. 25.9%, p =0.009). The GG genotype was particularly higher in patients with tuberculoid (TT) and borderline (BB) leprosy (90.5% and 89.8%, respectively). Analysis of IL10 genotypes revealed a lower frequency of GCC/GCC haplotype in lepromatous leprosy (LL) patients (6.2%) in comparison to controls (15.4%). Conclusion It is suggested that the G→A substitution at position −308 in the TNF promoter region plays an important role in leprosy patients. |
| Databáze: | OpenAIRE |
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