Enhanced drug delivery via hyperthermal membrane disruption using targeted gold nanoparticles with PEGylated Protein-G as a cofactor
| Autor: | Martin G. O’Toole, Xinghua Sun, Guandong Zhang, Robert S. Keynton, Dhruvinkumar Patel, Andre M. Gobin |
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| Rok vydání: | 2012 |
| Předmět: |
Materials science
Receptor ErbB-2 Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Bioengineering Antineoplastic Agents Breast Neoplasms Pharmacology Polyethylene Glycols Cell membrane Drug Delivery Systems Bacterial Proteins Cell Line Tumor medicine Humans General Materials Science Breast Surface plasmon resonance biology Cancer Hyperthermia Induced Photothermal therapy medicine.disease Recombinant Proteins medicine.anatomical_structure Colloidal gold Immunoglobulin G Cancer cell Drug delivery biology.protein Cancer research Molecular Medicine Nanoparticles Female Protein G Gold Laser Therapy Antibodies Immobilized |
| Zdroj: | Nanomedicine : nanotechnology, biology, and medicine. 9(8) |
| ISSN: | 1549-9642 |
| Popis: | Gold nanoparticles (GNPs) with near infrared (NIR) plasmon resonance have been promisingly used in photothermal cancer therapy as a less invasive treatment. Recombinant Protein-G (ProG) was PEGylated to act as a cofactor to immobilize immunoglobulins (IgGs) on GNPs by the F c region, resulting in optimal orientation of IgGs for efficient cancer targeting. In-vitro studies showed that HER-2 overexpressing breast cancer cells, SK-BR-3, were efficiently targeted and ablated at a laser power of 900 J/cm 2 (5 W/cm 2 for 3 min). However, as a means of enhancing treatment efficacy by increasing cellular sensitivity to chemotherapeutic agents, we showed that GNP exposure to lower power laser resulted in small disruptions of cell membrane due to localized hyperthermia. This did not lead to cell death but provided a mechanism for killing cancer cells by providing enhanced uptake of drug molecules thus leading to a new avenue for hyperthermia-anticancer drug combined cancer therapeutics. From the Clinical Editor PEGylated recombinant Protein-G was used as a cofactor to optimize the orientation of IgGs providing “target seeking” properties to gold nanoparticles used in photothermal cancer therapy. The system demonstrated excellent properties in cancer therapy, with the hope and expectation of future clinical translation. |
| Databáze: | OpenAIRE |
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