Members of the NuRD Chromatin Remodeling Complex Interact with AUF1 in Developing Cortical Neurons
| Autor: | Dragan Maric, Navid Sadri, Robert J. Schneider, Michael Lawson, Andrea Gyorgy, Denes V. Agoston, Cheol Lee, Jeffery L. Barker |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Cognitive Neuroscience
Molecular Sequence Data Biology SAP30 Transfection Histone Deacetylases Chromatin remodeling Immunophenotyping Cellular and Molecular Neuroscience Pregnancy Animals Histone code Heterogeneous Nuclear Ribonucleoprotein D0 Heterogeneous-Nuclear Ribonucleoprotein D DNA Primers Cerebral Cortex Genetics Histone deacetylase 5 Base Sequence Brain Gene Expression Regulation Developmental Articles Embryo Mammalian Flow Cytometry Mi-2/NuRD complex HDAC4 Chromatin Rats Cell biology Animals Newborn Histone deacetylase complex Female Mi-2 Nucleosome Remodeling and Deacetylase Complex |
| Zdroj: | Cerebral Cortex. 18:2909-2919 |
| ISSN: | 1460-2199 1047-3211 |
| DOI: | 10.1093/cercor/bhn051 |
| Popis: | Chromatin remodeling plays an important role in coordinating gene expression during cortical development, however the identity of molecular complexes present in differentiating cortical neurons that mediate the process remains poorly understood. The A + U–rich element-binding factor 1 (AUF1) is a known regulator of messenger RNA stability and also acts as a transcription factor upon binding to AT-rich DNA elements. Here we show that AUF1 is specifically expressed in subsets of proliferating neural precursors and differentiating postmitotic neurons of the developing cerebral cortex. Moreover, AUF1 is coexpressed with histone deacetylase 1 (HDAC1) and metastasis-associated protein 2 (MTA2), members of the nucleosome remodeling and histone deacetylase complex. AUF1 specifically and simultaneously binds to HDAC1, MTA2, and AT-rich DNA element, its gene regulatory function is modulated by the extent of histone acetylation and in animals lacking AUF1, the composition of the complex is modified. These results suggest that AUF1 is involved in integrating genetic and epigenetic signals during cortical development through recruiting HDAC1 and MTA2 to AT-rich DNA elements. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|