Herbaspirillum rubrisubalbicans, a mild pathogen impairs growth of rice by augmenting ethylene levels
| Autor: | Thiago Borba Kokot, Liziane Cristina Campos Brusamarello-Santos, Emanuel Maltempi de Souza, Caroline Kukolj, Dayane Alberton, Rose A. Monteiro, Fábio O. Pedrosa, Glaucio Valdameri, Vivian Rotuno Moure |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Herbaspirillum Iron Plant Science Reductase Biology Rhizobacteria medicine.disease_cause 01 natural sciences Plant Roots 03 medical and health sciences Bacterial Proteins Gene Expression Regulation Plant Botany Genetics medicine Type VI secretion system Plant Diseases Plant Proteins chemistry.chemical_classification Reactive oxygen species Effector food and beverages Oryza General Medicine Ethylenes biology.organism_classification 030104 developmental biology Biochemistry chemistry Herbaspirillum rubrisubalbicans Host-Pathogen Interactions Epiphytic bacteria Agronomy and Crop Science Oxidative stress 010606 plant biology & botany |
| Zdroj: | Plant molecular biology. 94(6) |
| ISSN: | 1573-5028 |
| Popis: | Herbaspirillum rubrisubalbicans decreases growth of rice. Inoculation of rice with H. rubrisubalbicans increased the ACCO mRNA levels and ethylene production. The H. rubrisubalbicans rice interactions were further characterized by proteomic approach. Herbaspirillum rubrisubalbicans is a well-known growth-promoting rhizobacteria that can also act as a mild phyto-pathogen. During colonisation of rice, RT-qPCR analyses showed that H. rubrisubalbicans up-regulates the methionine recycling pathway as well as phyto-siderophore synthesis genes. mRNA levels of ACC oxidase and ethylene levels also increased in rice roots but inoculation with H. rubrisubalbicans impaired growth of the rice plant. A proteomic approach was used to identify proteins specifically modulated by H. rubrisubalbicans in rice and amongst the differentially expressed proteins a V-ATPase and a 14-3-3 protein were down-regulated. Several proteins of H. rubrisubalbicans were identified, including the type VI secretion system effector Hcp1, suggesting that protein secretion play a role colonisation in rice. Finally, the alkyl hydroperoxide reductase, a primary scavenger of endogenous hydrogen peroxide was also identified. Monitoring the levels of reactive oxygen species in the epiphytic bacteria by flow cytometry revealed that H. rubrisubalbicans is subjected to oxidative stress, suggesting that the alkyl hydroperoxide reductase is an important regulator of redox homeostasis in plant-bacteria interactions. |
| Databáze: | OpenAIRE |
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