Determination of risperidone and its major metabolite 9-hydroxyrisperidone in human plasma by reversed-phase liquid chromatography with ultraviolet detection
| Autor: | Edoardo Spina, Gabriella Facciolà, Monica Salemi, Angela Avenoso |
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| Rok vydání: | 2000 |
| Předmět: |
Detection limit
Chromatography medicine.diagnostic_test Metabolite Reproducibility of Results General Chemistry Reversed-phase chromatography Isoxazoles Risperidone High-performance liquid chromatography Sensitivity and Specificity chemistry.chemical_compound Pyrimidines chemistry Pharmacokinetics Potassium phosphate Therapeutic drug monitoring Paliperidone Palmitate medicine Humans Spectrophotometry Ultraviolet Quantitative analysis (chemistry) Chromatography High Pressure Liquid Antipsychotic Agents |
| Zdroj: | Journal of chromatography. B, Biomedical sciences and applications. 746(2) |
| ISSN: | 1387-2273 |
| Popis: | A simple and sensitive high-performance liquid chromatographic (HPLC) method with UV absorbance detection is described for the quantitation of risperidone and its major metabolite 9-hydroxyrisperidone in human plasma, using clozapine as internal standard. After sample alkalinization with 1 ml of NaOH (2 M) the test compounds were extracted from plasma using diisopropyl ether-isoamylalcohol (99:1, v/v). The organic phase was back-extracted with 150 microl potassium phosphate (0.1 M, pH 2.2) and 60 microl of the acid solution was injected into a C18 BDS Hypersil analytical column (3 microm, 100x4.6 mm I.D.). The mobile phase consisted of phosphate buffer (0.05 M, pH 3.7 with 25% H3PO4)-acetonitrile (70:30, v/v), and was delivered at a flow-rate of 1.0 ml/min. The peaks were detected using a UV detector set at 278 nm and the total time for a chromatographic separation was about 4 min. The method was validated for the concentration range 5-100 ng/ml. Mean recoveries were 98.0% for risperidone and 83.5% for 9-hydroxyrisperidone. Intra- and inter-day relative standard deviations were less than 11% for both compounds, while accuracy, expressed as percent error, ranged from 1.6 to 25%. The limit of quantitation was 2 ng/ml for both analytes. The method shows good specificity with respect to commonly prescribed psychotropic drugs, and it has successfully been applied for pharmacokinetic studies and therapeutic drug monitoring. |
| Databáze: | OpenAIRE |
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