Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
Autor: | David Piquemal, Caroline Joubert, Laurent Manchon, Benjamin Marie, Fabien Pierrat, Yannick Gueguen, Isabelle Zanella-Cléon, Caroline Montagnani, Nathalie Cochennec-Laureau |
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Přispěvatelé: | Laboratoire de Biotechnologie et de Qualité de la Perle (LBQP), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Skuldtech. Cap Delta - ZAC Euromedecine II. 1682, rue de la Valsière, Molécules de Communication et Adaptation des Micro-Organismes (MCAM), Muséum national d'Histoire naturelle (MNHN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de Génétique Moléculaire de Montpellier (IGMM), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Skuld-Tech, biotechnology company (Skuld-Tech), Skuld-Tech, Institut de biologie et chimie des protéines [Lyon] (IBCP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Ecosystèmes Insulaires Océaniens (UMR 241) (EIO), Université de la Polynésie Française (UPF)-Institut Louis Malardé [Papeete] (ILM), Institut de Recherche pour le Développement (IRD)-Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Centre Océanologique du Pacifique (COP), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de la Polynésie Française (UPF)-Institut Louis Malardé [Papeete] (ILM), Institut de Recherche pour le Développement (IRD), Skuldtech, Biogéosciences [UMR 6282] [Dijon] (BGS), Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Institut Français de Recherche pour l'Exploitation de la Mer - Atlantique (IFREMER Atlantique), Study supported by the 'Service de la perliculture' of French Polynesia, Ifremer, Skuldtech and University of French Polynesia., Laboratoire de Biotechnologie et de Qualité de la Perle ( LBQP ), Institut Français de Recherche pour l'Exploitation de la Mer ( IFREMER ), Biogéosciences [Dijon] ( BGS ), AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université de Bourgogne ( UB ) -Centre National de la Recherche Scientifique ( CNRS ), Institut de biologie et chimie des protéines [Lyon] ( IBCP ), Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique ( CNRS ), Institut Français de Recherche pour l'Exploitation de la Mer - Nantes ( IFREMER Nantes ), Université de Nantes ( UN ) |
Jazyk: | angličtina |
Rok vydání: | 2010 |
Předmět: |
0106 biological sciences
Models Molecular Proteomics Proteome [SDV]Life Sciences [q-bio] 01 natural sciences Contig Mapping Mantle (mollusc) Margaritifera In Situ Hybridization Genetics Expressed Sequence Tags 0303 health sciences Minerals biology Pinctada margaritifera [ SDV.BBM.GTP ] Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] Biotechnology Research Article lcsh:QH426-470 Sequence analysis lcsh:Biotechnology Molecular Sequence Data 010603 evolutionary biology 03 medical and health sciences Calcification Physiologic lcsh:TP248.13-248.65 [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] Animals [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Amino Acid Sequence Pinctada RNA Messenger [SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials 030304 developmental biology Base Sequence Sequence Homology Amino Acid Gene Expression Profiling Animal Structures Molecular Sequence Annotation Sequence Analysis DNA biology.organism_classification [ SDV.IB.BIO ] Life Sciences [q-bio]/Bioengineering/Biomaterials lcsh:Genetics Gene Expression Regulation Evolutionary biology Biomineralization |
Zdroj: | Bmc Genomics (1471-2164) (Biomed Central Ltd), 2010-11, Vol. 11, P. 1-13 BMC Genomics BMC Genomics, BioMed Central, 2010, 11 (1), ⟨10.1186/1471-2164-11-613⟩ BMC Genomics, 2010, 11 (1), ⟨10.1186/1471-2164-11-613⟩ BMC Genomics, BioMed Central, 2010, 11, pp.613. ⟨10.1186/1471-2164-11-613⟩ BMC Genomics, BioMed Central, 2010, 11, pp.613. 〈10.1186/1471-2164-11-613〉 BMC Genomics, Vol 11, Iss 1, p 613 (2010) |
ISSN: | 1471-2164 |
Popis: | Background The shell of the pearl-producing bivalve Pinctada margaritifera is composed of an organic cell-free matrix that plays a key role in the dynamic process of biologically-controlled biomineralization. In order to increase genomic resources and identify shell matrix proteins implicated in biomineralization in P. margaritifera, high-throughput Expressed Sequence Tag (EST) pyrosequencing was undertaken on the calcifying mantle, combined with a proteomic analysis of the shell. Results We report the functional analysis of 276 738 sequences, leading to the constitution of an unprecedented catalog of 82 P. margaritifera biomineralization-related mantle protein sequences. Components of the current "chitin-silk fibroin gel-acidic macromolecule" model of biomineralization processes were found, in particular a homolog of a biomineralization protein (Pif-177) recently discovered in P. fucata. Among these sequences, we could show the localization of two other biomineralization protein transcripts, pmarg-aspein and pmarg-pearlin, in two distinct areas of the outer mantle epithelium, suggesting their implication in calcite and aragonite formation. Finally, by combining the EST approach with a proteomic mass spectrometry analysis of proteins isolated from the P. margaritifera shell organic matrix, we demonstrated the presence of 30 sequences containing almost all of the shell proteins that have been previously described from shell matrix protein analyses of the Pinctada genus. The integration of these two methods allowed the global composition of biomineralizing tissue and calcified structures to be examined in tandem for the first time. Conclusions This EST study made on the calcifying tissue of P. margaritifera is the first description of pyrosequencing on a pearl-producing bivalve species. Our results provide direct evidence that our EST data set covers most of the diversity of the matrix protein of P. margaritifera shell, but also that the mantle transcripts encode proteins present in P. margaritifera shell, hence demonstrating their implication in shell formation. Combining transcriptomic and proteomic approaches is therefore a powerful way to identify proteins involved in biomineralization. Data generated in this study supply the most comprehensive list of biomineralization-related sequences presently available among protostomian species, and represent a major breakthrough in the field of molluskan biomineralization. |
Databáze: | OpenAIRE |
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