RNA Footprinting Using Small Chemical Reagents
| Autor: | Bruno Sargueil, Grégoire De Bisschop |
|---|---|
| Přispěvatelé: | Cibles Thérapeutiques et conception de médicaments (CiTCoM - UMR 8038), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
0303 health sciences Chemistry RNA [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Ligand (biochemistry) Small molecule Footprinting 03 medical and health sciences 0302 clinical medicine Biophysics Nucleotide Binding site Nucleic acid structure 030217 neurology & neurosurgery ComputingMilieux_MISCELLANEOUS 030304 developmental biology Ribonucleoprotein |
| Zdroj: | RNA Scaffolds RNA Scaffolds, 2323, Springer US, pp.13-23, 2021, Methods in Molecular Biology, ⟨10.1007/978-1-0716-1499-0_2⟩ RNA Scaffolds ISBN: 9781071614983 |
| DOI: | 10.1007/978-1-0716-1499-0_2⟩ |
| Popis: | RNA is a pivotal element of the cell which is most of the time found in complex with protein(s) in a cellular environment. RNA can adopt three-dimensional structures that may form specific binding sites not only for proteins but for all sorts of molecules. Since the early days of molecular biology, strategies to probe RNA structure have been developed. Such probes are small molecules or RNases that most of the time specifically react with single strand nucleotides. The precise reaction or cleavage site can be mapped by reverse transcription. It appears that nucleotides in close contact or in proximity of a ligand are no longer reactive to these probes. Carrying the RNA probing experiment in parallel in presence and absence of a ligand yield differences that are known as the ligand "footprint." Such footprints allow for the identification of the precise site of the ligand interaction, but also reveals RNA structural rearrangement upon ligand binding. Here we provide an experimental and analytical workflow to carry RNA footprinting experiments. |
| Databáze: | OpenAIRE |
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