The complete genome of Rachiplusia nu nucleopolyhedrovirus (RanuNPV) and the identification of a baculoviral CPD-photolyase homolog
| Autor: | Ethiane Rozo dos Santos, Daniel R. Sosa-Gómez, Luana Beló Trentin, Daniel M. P. Ardisson-Araújo, Bergmann Morais Ribeiro, Admilton Gonçalves de Oliveira Junior |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Baculoviridae
food.ingredient viruses Genome Viral Moths Virus Open Reading Frames Viral Proteins 03 medical and health sciences food Rachiplusia nu Virology Chrysodeixis includens Betabaculovirus Animals ORFS Nucleocapsid Gene Phylogeny 030304 developmental biology Base Composition 0303 health sciences Base Sequence biology fungi 030302 biochemistry & molecular biology Virion biology.organism_classification Nucleopolyhedroviruses Alphabaculovirus Deoxyribodipyrimidine Photo-Lyase |
| Zdroj: | Virology. 534:64-71 |
| ISSN: | 0042-6822 |
| Popis: | We described a novel baculovirus isolated from the polyphagous insect pest Rachiplusia nu. The virus presented pyramidal-shaped occlusion bodies (OBs) with singly-embed nucleocapsids and a dose mortality response of 6.9 × 103 OBs/ml to third-instar larvae of R. nu. The virus genome is 128,587 bp long with a G + C content of 37.9% and 134 predicted ORFs. The virus is an alphabaculovirus closely related to Trichoplusia ni single nucleopolyhedrovirus, Chrysodeixis chalcites nucleopolyhedrovirus, and Chrysodeixis includens single nucleopolyhedrovirus and may constitute a new species. Surprisingly, we found co-evolution among the related viruses and their hosts at species level. Besides, auxiliary genes with homologs in other baculoviruses were found, e.g. a CPD-photolyase. The gene seemed to be result of a single event of horizontal transfer from lepidopterans to alphabaculovirus, followed by a transference from alpha to betabaculovirus. The predicted protein appears to be an active enzyme that ensures likely DNA protection from sunlight. |
| Databáze: | OpenAIRE |
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