Bacillus subtilis spores as delivery system for nasal Plasmodium falciparum circumsporozoite surface protein immunization in a murine model

Autor: Luis André Morais Mariúba, Gemilson Soares Pontes, Antônio Alcirley da Silva Balieiro, Juliane Corrêa Glória, Walter Luiz Lima Neves, Andréa Monteiro Tarragô, Maria Gabriella Santos de Vasconcelos, Spartaco Astolfi Filho, Ezio Ricca, Yury Oliveira Chaves, Maria Edilene Martins de Almeida, Rachele Isticato, Késsia Caroline Souza Alves, Júlio Nino de Souza Neto, Thiago Serrão Pinto
Přispěvatelé: de Almeida, M. E. M., Alves, K. C. S., de Vasconcelos, M. G. S., Pinto, T. S., Gloria, J. C., Chaves, Y. O., Neves, W. L. L., Tarrago, A. M., de Souza Neto, J. N., Astolfi-Filho, S., Pontes, G. S., da Silva Balieiro, A. A., Isticato, R., Ricca, E., Mariuba, L. A. M.
Rok vydání: 2022
Předmět:
Zdroj: Scientific Reports
Scientific Reports, Vol 12, Iss 1, Pp 1-8 (2022)
ISSN: 2045-2322
Popis: Malaria remains a widespread public health problem in tropical and subtropical regions around the world, and there is still no vaccine available for full protection. In recent years, it has been observed that spores of Bacillus subtillis can act as a vaccine carrier and adjuvant, promoting an elevated humoral response after co-administration with antigens either coupled or integrated to their surface. In our study, B. subtillis spores from the KO7 strain were used to couple the recombinant CSP protein of P. falciparum (rPfCSP), and the nasal humoral-induced immune response in Balb/C mice was evaluated. Our results demonstrate that the spores coupled to rPfCSP increase the immunogenicity of the antigen, which induces high levels of serum IgG, and with balanced Th1/Th2 immune response, being detected antibodies in serum samples for 250 days. Therefore, the use of B. subtilis spores appears to be promising for use as an adjuvant in a vaccine formulation.
Databáze: OpenAIRE
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