LncRNA SNHG20 promotes migration and invasion of ovarian cancer via modulating the microRNA-148a/ROCK1 axis
| Autor: | Yu-Jie Dong, Qi Yang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Clone (cell biology) SNHG20 Mice Nude Apoptosis Biology Cell Line Flow cytometry Metastasis Cell Movement Ovarian cancer ROCK1 microRNA microRNA-148a medicine Animals Humans Neoplasm Invasiveness Ovarian Neoplasms Mice Inbred BALB C rho-Associated Kinases medicine.diagnostic_test Cell growth Research Obstetrics and Gynecology Gynecology and obstetrics medicine.disease MicroRNAs Oncology Cancer research RG1-991 Female RNA Long Noncoding |
| Zdroj: | Journal of Ovarian Research, Vol 14, Iss 1, Pp 1-13 (2021) Journal of Ovarian Research |
| ISSN: | 1757-2215 |
| Popis: | BackgroundOvarian cancer (OC) is characterized by early metastasis and poor prognosis, which threatens the health of women worldwide. Small nucleolar RNA host gene 20 (SNHG20), a long noncoding RNA (lncRNA), has been verified to be significantly up-regulated in several tumors, including OC. MicroRNA-148a (miR-148a)/rho-kinase1 (ROCK1) axis plays an important role in the modulation of tumor development. However, whether SNHG20 can regulate OC progression through miR-148a/ROCK1 axis remains unclear. Normal human ovarian epithelial cell line and four OC cell lines were adopted for in vitro experiments. Real-time PCR was performed to assess the levels of SNHG20 and miR-148a. OC cell proliferation, apoptosis, invasion and migration were detected using clone formation, flow cytometry, transwell, and wound healing assays, respectively. Tumor xenograft assay was applied to evaluate the effect of SNHG20 on tumor growth in vivo.ResultsSignificant higher expression of SNHG20 was observed in OC cell lines. SNHG20 markedly promoted the invasion, migration, proliferation and inhibited the apoptosis of OC cells. SNHG20 enhanced ROCK1 expression by sponging miR-148a, and the direct binding between SNHG20/ROCK1 and miR-148a was identified.ConclusionSNHG20 promoted invasion and migration of OC via targeting miR-148a/ROCK1 axis. The present research may provide a novel insight for the therapeutic strategies of OC. |
| Databáze: | OpenAIRE |
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