Interleukin-37 Expression Is Increased in Chronic HIV-1-Infected Individuals and Is Associated with Inflammation and the Size of the Total Viral Reservoir

Autor: Ole S. Søgaard, Mayumi Fujita, Anni Winckelmann, Holger Jon Møller, Karen Lise Dahl Andersen, Jesper Damsgaard Gunst, Rune R. Laursen, Lars Østergaard, Jesper F Højen, Charles A. Dinarello, Martin Tolstrup, Thomas A Rasmussen
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Adult
Lipopolysaccharides
Male
medicine.medical_treatment
lnfectious Diseases and Global Health Radboud Institute for Molecular Life Sciences [Radboudumc 4]
Lipopolysaccharide Receptors
Antigens
Differentiation
Myelomonocytic
Gene Expression
Inflammation
HIV Infections
Receptors
Cell Surface
Lymphocyte Activation
Peripheral blood mononuclear cell
Flow cytometry
Antigens
CD
Antiretroviral Therapy
Highly Active
Genetics
medicine
Humans
RNA
Messenger
Interleukin 6
Molecular Biology
Genetics (clinical)
medicine.diagnostic_test
biology
Interleukin-6
Monocyte
Interleukin
Articles
Middle Aged
Viral Load
CD4 Lymphocyte Count
medicine.anatomical_structure
Cytokine
Cross-Sectional Studies
Immunology
biology.protein
Leukocytes
Mononuclear
Molecular Medicine
Female
medicine.symptom
Viral load
Biomarkers
Interleukin-1
Zdroj: Molecular Medicine, 21, pp. 337-45
Hansen, J F, Rasmussen, T A, Andersen, K L D, Winckelmann, A A, Laursen, R R, Damsgaard Gunst, J, Møller, H J, Fujita, M, Østergaard, L J, Søgaard, O S, Dinarello, C A & Tolstrup, M 2015, ' Interleukin-37 expression is increased in chronic HIV-1-infected individuals and is associated with inflammation and the size of the total viral reservoir ', Molecular Medicine . https://doi.org/10.2119/molmed.2015.00031
Molecular Medicine, 21, 337-45
ISSN: 1076-1551
Popis: Contains fulltext : 153198.pdf (Publisher’s version ) (Open Access) Interleukin-37 (IL-37) is a recently identified cytokine with potent antiinflammatory and immunosuppressive functions. The objective of this study was to compare levels of IL-37 mRNA in immunological subgroups of chronic human immunodeficiency virus-1 (HIV-1)-infected individuals and noninfected controls, to determine IL-37's association with biomarkers of inflammation and reservoir size. This was a cross-sectional study. The HIV-1-infected patients were categorized in three subgroups depending on their combination antiretroviral therapy (cART) treatment status and CD4(+) T-cell count. Quantitative RT-PCR was used for the detection of IL-37 mRNA and HIV-1 DNA in peripheral blood mononuclear cells (PBMCs). Biomarkers in plasma were quantified by enzyme-linked immunosorbent assay (ELISA), whereas T-cell activation was determined by flow cytometry. Lastly, lipopolysaccharide (LPS) stimulations of patients PBMCs were carried out to determine differences in IL-37 mRNA response between the subgroups. Sixty HIV-1-infected patients and 20 noninfected controls were included in the study. Steady-state IL-37 mRNA levels in PBMCs were significantly higher in HIV-1-infected individuals compared with noninfected controls: 2.4-fold (p
Databáze: OpenAIRE
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