The Effect of Chyle Fat Injection on Human Hypertrophic Scars in an Animal Model
| Autor: | Minliang Chen, Linying Lai, Yunchao Zhou, Guiwen Zhou, Xiao Xu, Junnan Chen, Kui Ma, Zhenya Huang, Liming Liang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Male
medicine.medical_specialty Time Factors Chyle Cicatrix Hypertrophic Decorin Mice Nude Injections Intralesional 030230 surgery Triamcinolone Risk Assessment Gastroenterology Masson's trichrome stain Mice Random Allocation 03 medical and health sciences Hypertrophic scar 0302 clinical medicine Nude mouse Internal medicine Adipocytes Animals Humans Medicine Wound Healing biology business.industry Therapeutic effect Histology biology.organism_classification medicine.disease Staining Disease Models Animal Treatment Outcome Adipose Tissue 030220 oncology & carcinogenesis Tissue and Organ Harvesting Surgery Burns business Follow-Up Studies |
| Zdroj: | Annals of Plastic Surgery. 82:622-627 |
| ISSN: | 1536-3708 0148-7043 |
| DOI: | 10.1097/sap.0000000000001784 |
| Popis: | BACKGROUND Chyle fat transplantation has shown positive effects on preexisting human hypertrophic scars (HSs) in a nude mouse HS graft model. METHODS Hypertrophic scar fragments were obtained from 5 surgically treated burn patients and implanted into the backs of nude mice in 3 groups: group A, control; group B, triamcinolone; and group C, chyle fat. The specimens were implanted after the corresponding intralesional injection in each group, and the mice were observed for 4 weeks. In total, 18 mice and 72 scar specimens were studied. After 4 weeks, the HSs were removed from the mice. Then, the scar weights, histology, and decorin staining were assessed to evaluate the therapeutic efficacy. RESULTS An obviously significant difference was observed in the HS weight reduction between groups A and C (P < 0.01), and a significant difference in the HS weight reduction was observed between groups A and B (P < 0.05). However, there was no significant difference between groups B and C. The treatment groups (groups B and C) showed strong decorin staining. Furthermore, the decorin staining was much stronger in group C than in group B (P < 0.05). Significant differences in extracellular matrix deposition were observed among the 3 groups, as determined by Masson trichrome staining. Both groups B and C showed significant therapeutic efficacy compared with group A, and group C exhibited a significant therapeutic effect compared with group B (P < 0.05). CONCLUSIONS This study indicates that chyle fat grafting is beneficial for treating HSs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|