The smfBox is an open-source platform for single-molecule FRET
| Autor: | Benjamin Ambrose, Matthew Willmott, Ashley J. Cadby, Marisa L. Martin-Fernandez, James Baxter, Marleen Aaldering, Elliot Steele, Jonathan R. Shewring, Benji C. Bateman, Timothy D. Craggs, John Cully |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Multidisciplinary business.industry Computer science Science General Physics and Astronomy General Chemistry Single-molecule FRET General Biochemistry Genetics and Molecular Biology Article Computational science 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Förster resonance energy transfer Software Open source Single-molecule biophysics Fluorescence resonance energy transfer lcsh:Q lcsh:Science business 030217 neurology & neurosurgery |
| Zdroj: | Nature Communications Nature Communications, Vol 11, Iss 1, Pp 1-6 (2020) |
| ISSN: | 2041-1723 |
| Popis: | Single-molecule Förster Resonance Energy Transfer (smFRET) is a powerful technique capable of resolving both relative and absolute distances within and between structurally dynamic biomolecules. High instrument costs, and a lack of open-source hardware and acquisition software have limited smFRET’s broad application by non-specialists. Here, we present the smfBox, a cost-effective confocal smFRET platform, providing detailed build instructions, open-source acquisition software, and full validation, thereby democratising smFRET for the wider scientific community. Broad uptake of smFRET has been hindered by high instrument costs and a lack of open-source hardware and acquisition software. Here, the authors present the smfBox, a cost-effective open-source platform capable of measuring precise FRET efficiencies between dyes on freely diffusing single molecules. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|