Exposure of zebrafish larvae to water accommodated fractions of weathered crude oil alters steroid hormone concentrations with minimal effect on cholesterol
Autor: | Edward M. Mager, Daniel Schlenk, Aaron P. Roberts, Victoria McGruer, Fabrizio Bonatesta, Edwin R. Price |
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Rok vydání: | 2021 |
Předmět: |
endocrine system
medicine.medical_specialty animal structures Health Toxicology and Mutagenesis medicine.medical_treatment Aquatic Science Steroid chemistry.chemical_compound Corticosterone Internal medicine medicine Animals Petroleum Pollution Androstenedione Polycyclic Aromatic Hydrocarbons Testosterone Zebrafish Cholesterol fungi Water Hormones Steroid hormone Endocrinology Petroleum chemistry Dihydrotestosterone Larva embryonic structures Steroids Water Pollutants Chemical medicine.drug Hormone |
Zdroj: | Aquatic toxicology (Amsterdam, Netherlands). 242 |
ISSN: | 1879-1514 |
Popis: | Crude oil has multiple toxic effects in fish, particularly early life stages. Recent transcriptomics studies have highlighted a potential effect on cholesterol homeostasis and biosynthesis, but have not investigated effects on steroid hormones, which are biosynthetically downstream metabolites of cholesterol. We exposed zebrafish (Danio rerio) embryos and larvae to 3 concentrations of a high energy water accommodated fraction (HEWAF) of crude oil and measured effects on cholesterol and steroid hormones at 48 and 96 hours post fertilization (hpf). HEWAF exposure caused a small decrease in cholesterol at 96 hpf but not 48 hpf. HEWAF-exposed larvae had higher levels of androstenedione, testosterone, estradiol, cortisol, corticosterone, and progesterone at 96 hpf compared to controls, while effects at 48 hpf were more modest or not present. 2-Methoxyestradiol was lower following HEWAF exposure at both time points. Dihydrotestosterone was elevated in one HEWAF concentration at 48 hpf only. Our results suggest that hormone imbalance may be an important toxic effect of oil HEWAF exposure despite no major effect on their biosynthetic precursor cholesterol. |
Databáze: | OpenAIRE |
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