Possible involvement of a L-delta 1-pyrroline-5-carboxylate (P5C) reductase in the synthesis of proline in Desulfovibrio desulfuricans Norway
Autor: | Brigitte Cami, Michel Fons, Marc Chippaux |
---|---|
Rok vydání: | 1991 |
Předmět: |
DNA
Bacterial Hot Temperature Genotype Proline Mutant Biophysics Gene Expression Reductase medicine.disease_cause Biochemistry chemistry.chemical_compound Plasmid Biosynthesis Enzyme Stability medicine Escherichia coli Pyrroles Molecular Biology chemistry.chemical_classification biology Cell Biology biology.organism_classification Desulfovibrio Enzyme chemistry Plasmids |
Zdroj: | Biochemical and biophysical research communications. 179(2) |
ISSN: | 0006-291X |
Popis: | A L-delta 1-pyrroline-5-carboxylate reductase activity has been detected in crude extracts of Desulfovibrio desulfuricans Norway. This P5C reductase activity is also found when a 2.5 kb D. desulfuricans DNA fragment is introduced into an Escherichia coli proC mutant. Although it restores growth of the proC mutant, the ProDd enzyme might be detrimental to the E. coli host since the plasmid carrying the cognate proDd gene is segregated at high rate by the cells but is stabilized by small deletions which lead to a loss of the P5C reductase activity. |
Databáze: | OpenAIRE |
Externí odkaz: |