Characterization of two sucrase activites in Bacillus subtilis marburg
| Autor: | F. Kunst, Martial Pascal, R. Dedonder, Jean-Antoine Lepesant |
|---|---|
| Rok vydání: | 1971 |
| Předmět: |
Immunodiffusion
Sucrose Bacillus subtilis Biochemistry Sucrase chemistry.chemical_compound Transferases medicine Trypsin Enzyme inducer chemistry.chemical_classification Chromatography biology Molecular mass Levansucrase General Medicine Hydrogen-Ion Concentration biology.organism_classification Molecular biology Culture Media Molecular Weight Glucose Enzyme chemistry Enzyme Induction biology.protein medicine.drug |
| Zdroj: | Biochimie. 53:1059-1066 |
| ISSN: | 0300-9084 |
| DOI: | 10.1016/s0300-9084(71)80193-1 |
| Popis: | Summary Two proteins responsible for sucrase activity in Bacillus subtilis Marburg have been separated by chromatography on hydroxyapatite. These enzymes have been characterized as transfructosidases, namely a levansucrase and a sucrase. Their molecular weights are very similar (40,000). These enzymes differ by their catalytic properties, their sensitivity to trypsin and SH reagents and by their immunological properties. They are both induced by sucrose ; only sucrase synthesis is decreased in the presence of glucose. Sucrase is endocellular while levansucrase is excreted in the culture medium. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|