A PCR for the detection of mycoplasmas belonging to the Mycoplasma mycoides cluster : application to the diagnosis of contagious agalactia

Autor: Lucia Manso-Silvan, François Poumarat, Marie-Pierre Pellet, François Thiaucourt, Patrice Gaurivaud, Sophie Lorenzon, V.P. Singh, Salah Woubit
Přispěvatelé: Contrôle des maladies animales exotiques et émergentes ( Contrôle des maladies ), Centre de Coopération Internationale en Recherche Agronomique pour le Développement ( CIRAD ), Mycoplasmoses des ruminants [Lyon], Université de Lyon-VetAgro Sup ( VAS ), Agence Française de Sécurité Sanitaire des Aliments ( AFSSA ), AFSSA, IVRI, National Referral Lab on Mycoplasma, Contrôle des maladies animales exotiques et émergentes (Cirad-EMVT-UPR 15 Contrôle des maladies), Département Elevage et médecine vétérinaire (EMVT), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Mycoplasmoses des Ruminants - UMR (MYCO), Université de Lyon-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Agence Française de Sécurité Sanitaire des Aliments (AFSSA), Département Elevage et médecine vétérinaire (Cirad-EMVT)
Jazyk: angličtina
Rok vydání: 2007
Předmět:
MESH : Molecular Sequence Data
MESH : Operon
Hydrolases
Mycoplasma agalactiae
ved/biology.organism_classification_rank.species
Pcr cloning
MESH: Base Sequence
[ SDV.MP.BAC ] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
medicine.disease_cause
Polymerase Chain Reaction
Mycoplasma capricolum
Serology
0403 veterinary science
MESH : Hydrolases
MESH: Animals
MESH: Phylogeny
MESH: Pleuropneumonia
Contagious
MESH : Polymerase Chain Reaction
Base Pairing
Phylogeny
0303 health sciences
biology
XUMYCOPLASME
MESH : Sequence Alignment
Goats
Mycoplasma mycoides
[ SDV.SPEE ] Life Sciences [q-bio]/Santé publique et épidémiologie
04 agricultural and veterinary sciences
3. Good health
MESH: Hydrolases
MESH: Reproducibility of Results
MESH : Pleuropneumonia
Contagious
Milk
MESH: Mycoplasma mycoides
MESH : DNA Primers
MESH: DNA Primers
MESH: Operon
040301 veterinary sciences
MESH: Base Pairing
Molecular Sequence Data
MESH: Sequence Alignment
MESH: Goats
MYCOPLASME
Microbiology
03 medical and health sciences
Operon
medicine
Animals
L50 - Physiologie et biochimie animales
Pleuropneumonia
Contagious
Molecular Biology
Gene
DNA Primers
MESH: Molecular Sequence Data
[SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health
Base Sequence
030306 microbiology
ved/biology
MESH : Reproducibility of Results
MESH : Phylogeny
Reproducibility of Results
MESH: Polymerase Chain Reaction
Cell Biology
Mycoplasma
biology.organism_classification
MESH : Base Pairing
Virology
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
MESH: Milk
MESH : Goats
MESH : Milk
HAFSSA
Mycoplasma putrefaciens
[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie
MESH : Base Sequence
MESH : Animals
[ SDV.BA.MVSA ] Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health
MESH : Mycoplasma mycoides
Sequence Alignment
Zdroj: Molecular and Cellular Probes
Molecular and Cellular Probes, Elsevier, 2007, 21 (5-6), pp.391-9. 〈10.1016/j.mcp.2007.05.008〉
Molecular and Cellular Probes, Elsevier, 2007, 21 (5-6), pp.391-9. ⟨10.1016/j.mcp.2007.05.008⟩
ISSN: 0890-8508
1096-1194
DOI: 10.1016/j.mcp.2007.05.008〉
Popis: Contagious agalactia is a mycoplasmal infection caused by Mycoplasma agalactiae, Mycoplasma mycoides subsp. mycoides LC, M. mycoides subsp. capri, Mycoplasma capricolum subsp. capricolum and Mycoplasma putrefaciens. Identification of the causative organisms is usually performed by isolation and classical biochemical and serological tests, though this is a lengthy and cumbersome process for mycoplasmas. Specific PCR assays have been developed for the identification of Mycoplasma agalactiae and M. putrefaciens. For members of the M. mycoides cluster existing PCR tests are based on the amplification of highly conserved genes coding for ribosomal proteins, hence a possibility of cross-reactions. The gene glk, coding for a glucokinase, that is found in this cluster is very distantly related to any other bacterial glucokinase described so far. It was therefore chosen as target to design a new PCR test. The validation was performed independently in three laboratories in France and India using over 100 mycoplasma strains of various geographical origins. All strains belonging to the M. mycoides cluster were detected by amplification of the expected PCR product (428 bp) while no amplification was obtained from M. agalactiae strains. Our results demonstrate the universality of this PCR in spite of the great heterogeneity found within this cluster. This new tool may be of great help for the implementation of control measures directed towards contagious agalactia. © 2007 Elsevier Ltd All rights reserved.
Databáze: OpenAIRE
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