High level nitrosamines in rat faeces with colorectal cancer determined by a sensitive GC-MS method
| Autor: | Zhe Lin Xia, Shan Ming Tian, Zhen Xiong Zhao, Sai Zhen Chen, Qing Fan, Ling Ling Zhang, Yu Bin Xu |
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| Rok vydání: | 2021 |
| Předmět: |
Analyte
Chromatography Nitrosamines Chemistry Clinical Biochemistry Extraction (chemistry) Pharmaceutical Science Pilot Projects Mass spectrometry Gas Chromatography-Mass Spectrometry Analytical Chemistry Rats Standard curve chemistry.chemical_compound Feces In vivo Tandem Mass Spectrometry Drug Discovery Animals Gas chromatography–mass spectrometry Nitrite Colorectal Neoplasms Spectroscopy |
| Zdroj: | Journal of pharmaceutical and biomedical analysis. 210 |
| ISSN: | 1873-264X |
| Popis: | N-nitrosamines (NAs) are common toxic substances that have a strong correlation with many human diseases, such as liver damage and cancer. However, there is a lack of studies on methods involving the detection of NAs in biological samples, possibly owing to the interference of complex biological matrices and the influence of endogenous NAs. In this work, solid-phase extraction with mixed solid phases and adsorption sedimentation were used to successfully establish a gas chromatography-mass spectrometry (GC-MS) method for detecting eight NAs in rat faeces. Chromatographic separation of analytes was performed with Agilent VF-WAXms (30 m × 0.25 mm, 0.25 µm) GC columns. The LLOQs of eight NAs were set to the concentration of 0.5 ng/g and the obtained standard curves were linear, and correlation coefficients (r) were ≥ 0.99 for samples with concentration ranges of 0.5-500 ng/g. The inter and intra-assay precisions were 15% for all analytes in the quality control samples, and the accuracies ranged from 88.67% to 108.33%. The extraction recoveries were above 78.56% for seven NAs, and a significant matrix effect was not observed. The application of this method revealed that the levels of NAS in the faeces of rats with colorectal cancer were higher than those of normal rats. Additionally, the effect of a high nitrite diet on NAs in faeces was analysed; the results confirmed that a high nitrite diet might contribute to an abnormal increase in NAs. Our work provides an analytical method for further in vivo study of NAs. Furthermore, a pilot study on the relationship between NAs and colorectal cancer was completed. |
| Databáze: | OpenAIRE |
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