Chromosome screening using culture medium of embryos fertilised in vitro: a pilot clinical study
| Autor: | Yao Bing, Fang Xiong, Li Chen, Liyi Cai, Weimin Yang, Caiqing Guo, Jianping Xiao, Xiaoqing Song, Xin Zhao, Honghua Wang, Xiao Xiao, Rui Fang, Jie Chen |
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| Rok vydání: | 2019 |
| Předmět: |
Adult
0301 basic medicine medicine.medical_treatment lcsh:Medicine Aneuploidy Pilot Projects Chromosomal ploidy Fertilization in Vitro Chromosomal rearrangement Biology General Biochemistry Genetics and Molecular Biology Intracytoplasmic sperm injection Andrology Young Adult 03 medical and health sciences 0302 clinical medicine Pregnancy medicine Chromosomes Human Humans Blastocyst Non-invasive chromosome screening In vitro fertilisation Clinical outcome Research lcsh:R Pregnancy Outcome Embryo culture Karyotype General Medicine Assisted reproductive technology Embryo Mammalian medicine.disease Culture Media 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis embryonic structures Next-generation sequencing Female Ploidy |
| Zdroj: | Journal of Translational Medicine Journal of Translational Medicine, Vol 17, Iss 1, Pp 1-8 (2019) |
| ISSN: | 1479-5876 |
| Popis: | Background Previous studies from this as well as other research groups suggested that non-invasive chromosome screening (NICS) with embryo culture medium can be used to identify chromosomal ploidy and chromosomal abnormalities. We here report a series of clinical cases utilizing the technology. Methods A total of 45 couples underwent in vitro fertilisation during a period between February 2016 and February 2017. Karyotyping revealed normal chromosomes in both partners in 23 couples, and chromosomal rearrangements in at least one partner in 22 couples. Intracytoplasmic sperm injection (ICSI) was used for fertilization. NICS was carried out using embryo culture medium at the blastocyst stage via multiple annealing and looping-based amplification cycles, whole-genome amplification and next-generation sequencing. Results A total of 413 embryos were obtained; 170 blastocysts were subjected to NICS. The screening showed euploidy in 79 embryos, aneuploidy in 52 embryos, and mosaic ploidy for 33 embryos. The rate of euploidy was comparable in couples with normal karyotype (50.7%; 38/75) vs. chromosomal rearrangement (43.2%; 41/95). A total of 52 euploid embryos (50 oocyte retrieval cycles) were transferred in 43 women. Biochemical pregnancy rate was 72.0% (36/50). Clinical pregnancy rate was 58.0% (29/50). The rate of spontaneous miscarriage was 3/29 (none with chromosomal aneuploidy). A total of 27 healthy babies were delivered. Conclusions NICS could identify embryo chromosomal abnormalities in couples either with or without chromosomal rearrangement, with satisfying clinical outcomes. Electronic supplementary material The online version of this article (10.1186/s12967-019-1827-1) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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