In vitro pharmacoregulation of CC chemokine ligand 5 and its receptor CCR5 in diffuse lung diseases
Autor: | Daniela Subrtova, Agata Gibejova, Frantisek Mrazek, Veronika Sekerova, Vitezslav Kolek, Roland M. du Bois, Martin Petrek |
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Jazyk: | angličtina |
Rok vydání: | 2003 |
Předmět: |
Adult
Lung Diseases Male CCR2 Leukocyte migration Pathology medicine.medical_specialty Sarcoidosis Receptors CCR5 Immunology Biology CCL5 Dexamethasone RANTES Cyclosporin a lcsh:Pathology medicine Leukocytes CCL17 Humans RNA Messenger Enzyme Inhibitors Pentoxifylline Receptor Chemokine CCL5 Glucocorticoids Bronchoalveolar cells Cells Cultured Aged Lung Tumor Necrosis Factor-alpha virus diseases Cell Biology respiratory system Middle Aged Ligand (biochemistry) respiratory tract diseases Cyclosporin A medicine.anatomical_structure Chemokines CC Cancer research Cyclosporine Female Bronchoalveolar Lavage Fluid Immunosuppressive Agents lcsh:RB1-214 Research Article |
Zdroj: | Mediators of Inflammation Mediators of Inflammation, Vol 12, Iss 4, Pp 215-220 (2003) |
ISSN: | 0962-9351 |
Popis: | Background:CC chemokine ligand (CCL)5 and its receptor CCR5 contribute to leukocyte migration into lungs of patients with diffuse lung diseases (DLD). Pharmacological regulation of CCL5 and CCR5 expression was therefore explored in bronchoalveolar cells obtained from patients with DLD.Methods:Cells from 21 patients were co-cultivatedin vitrowith tumour necrosis factor-α and dexamethasone, cyclosporin A (CyA) or pentoxifylline. Chemokine mRNA expression and protein production was assessed by reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay, respectively.Results:Dexamethasone altered CCL5 mRNA expression and suppressed its protein levels. CyA inhibited chemokine mRNA expression but not protein production. Pentoxifylline did not affected chemokine expression. Both dexamethasone and CyA suppressed CCR5 mRNA transcripts.Conclusion:In conclusion, while dexamethasone downregulates the CCL5 functional form, CyA and pentoxifylline have no effects on CCL5 protein. These data providein vitrocorrelation for clinical applications of immunomodulators in therapy of DLD. |
Databáze: | OpenAIRE |
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