TSH/miR-17-5p/ZNF367 axis is related to spontaneous abortion in patients with TSH above 2.5 mIU/L
| Autor: | Wen Dai, Weiping Teng, Yang Yang, Yingying Zhou, Jiashu Li, Zhongyan Shan |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Adult
endocrine system medicine.medical_specialty Kruppel-Like Transcription Factors Thyrotropin Abortion Pathogenesis Endocrinology Pregnancy Risk Factors Internal medicine microRNA medicine Humans In patient Molecular Biology 3' Untranslated Regions reproductive and urinary physiology business.industry Mir 17 5p Gene Expression Profiling Trophoblast Trophoblasts Abortion Spontaneous MicroRNAs medicine.anatomical_structure Increased risk Gene Expression Regulation ROC Curve Case-Control Studies embryonic structures Chorionic villi Female RNA Interference Disease Susceptibility business Biomarkers |
| Zdroj: | Journal of molecular endocrinology. 67(3) |
| ISSN: | 1479-6813 |
| Popis: | Elevated thyroid-stimulating hormone (TSH) is associated with an increased risk of spontaneous abortion (SA); however, the associated mechanism remains unclear. This study aimed to investigate the expression of miRNAs and pathogenesis in the chorionic villi of TSH > 2.5 mIU/L-related SA patients. The chorionic villi were collected from pregnant women in the first trimester with TSH > 2.5 mIU/L with or without SA, as well as TSH < 2.5 mIU/L with or without SA to determine the level of miRNA expression. Differentially expressed miRNAs were confirmed by qRT-PCR in a total of 92 subjects. Cell Counting Kit-8 (CCK8), wound healing, transwell assays, and Western blotting were used to measure cellular biological functions and related proteins in HTR-8/SVneo cells. The potential mechanisms were determined using a Luciferase reporter assay and rescue experiment. Compared with normal pregnant women, miR-17-5p was decreased and zinc finger protein 367 (ZNF367) was upregulated in the chorionic villi of TSH > 2.5 mIU/L-related SA patients. Using HTR-8/SVneo cells, we demonstrated that elevated TSH inhibited miR-17-5p expression, as well as trophoblast migration and invasion. The overexpression of miR-17-5p targeted and inhibited ZNF367 expression promoting the biological function of trophoblasts. Further studies confirmed that ZNF367 interference partially reversed the biological function of the miR-17-5p inhibitor on HTR-8/SVneo cells. Taken together, our results showed that miR-17-5p promoted the biological function of trophoblasts by suppressing ZNF367. |
| Databáze: | OpenAIRE |
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