Assembly of AKAP82, a protein kinase A anchor protein, into the fibrous sheath of mouse sperm
| Autor: | James A. Foster, Stuart B. Moss, Lisa Haig-Ladewig, Linda R. Johnson, Charles S. Rubin, Heidi Vanscoy, George L. Gerton |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Axoneme
Male endocrine system Protein subunit Immunoelectron microscopy Blotting Western Flagellum Biology sperm fibrous sheath Mice AKAP medicine Animals Phosphorylation Protein Precursors Protein kinase A Molecular Biology mouse Sperm flagellum Spermatid urogenital system Immune Sera Seminal Plasma Proteins Proteins Cell Biology Sperm Molecular biology Cyclic AMP-Dependent Protein Kinases Spermatids Peptide Fragments Molecular Weight medicine.anatomical_structure Solubility Sperm Tail Protein Processing Post-Translational Developmental Biology |
| Zdroj: | Developmental biology. 192(2) |
| ISSN: | 0012-1606 |
| Popis: | The assembly of the mammalian sperm flagellum is a complex developmental event requiring the sequential activation of genes encoding the component parts and the coordinated assembly of these proteins during the differentiation of the haploid spermatid. In this study, the mechanism underlying the assembly of the fibrous sheath surrounding the axoneme was examined. The subject of the study was the major fibrous sheath protein of the mouse sperm flagellum, AKAP82, a member of the A Kinase Anchor Protein (AKAP) family of polypeptides that bind the regulatory (RII) subunit of protein kinase A (PK-A). Immunoelectron microscopy demonstrated that AKAP82 is present throughout the transverse ribs and longitudinal columns of the fibrous sheath. Since AKAP82 is initially synthesized as a precursor (pro-AKAP82) during spermiogenesis, an antiserum was raised against a peptide from the processed region of pro-AKAP82 (M(r) 97,000). In immunoblotting experiments, the antibody detected pro-AKAP82 in condensing spermatids but not in epididymal sperm. In addition, two other immunoreactive proteins of M(r) 109,000 (p109) and M(r) 26,000 (p26, representing the "pro" domain of the precursor) were present in epididymal sperm. Alkaline phosphatase treatment of epididymal sperm proteins demonstrated that p109 was a phosphorylated form of pro-AKAP82 that remained in sperm. By immunofluorescence, pro-AKAP82 was localized to the entire length of the principal piece in testicular sperm, while in epididymal sperm p109 and p26 were present only in the proximal portion of the principal piece. Pro-AKAP82 was solubilized when germ cells were extracted with Triton X-100. However, in sperm, both AKAP82 and p109 were almost totally resistant to these extraction conditions and remained in the particulate fraction even after extraction with Triton and dithiothreitol. Similar to pro-AKAP82, the RII subunit of PK-A was present in the Triton X-100-soluble fraction of developing germ cells. In sperm, much of the RII also became particulate, consistent with the hypothesis that AKAP82 anchors RII in the flagellum. These data indicate that pro-AKAP82 is synthesized in the cell body, transported down the axoneme to its site of assembly in the fibrous sheath, and then proteolytically clipped to form mature AKAP82. |
| Databáze: | OpenAIRE |
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