Mammalian testes in organ culture
| Autor: | William H. Perloff, Emil Steinberger, Anna Steinberger |
|---|---|
| Rok vydání: | 1964 |
| Předmět: |
Male
Cell type Biology Organ culture Tissue Culture Techniques Andrology Tissue culture Organ Culture Techniques Testis medicine Animals Humans Mitosis Incubation Mammals Sertoli Cells Research Temperature Cell Biology Carbon Dioxide Hydrogen-Ion Concentration Sertoli cell Rats Oxygen Chemically defined medium medicine.anatomical_structure Immunology |
| Zdroj: | Experimental Cell Research. 36:19-27 |
| ISSN: | 0014-4827 |
| DOI: | 10.1016/0014-4827(64)90156-9 |
| Popis: | Testes of 14-day-old rats were grown as organ cultures for periods of up to 6 months. The effect of various culture media, gas phases, pH's and incubation temperatures was investigated. Tubular structure with healthy Sertoli cells and mitoses was well maintained under various culture conditions, including growth in chemically defined medium. Spermatocytes survived approximately four weeks without differentiating into spermatids. A cell type, resembling primitive germ cells of younger animals, consistently appeared in seminiferous tubules on the fourth day of culture. Since these cells are not normally observed in the testes of 14-day-old rats, their possible origin in culture is discussed. |
| Databáze: | OpenAIRE |
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