Isolation and characterization of chitinolytic bacterium, Escherichia fergusonii AMC01 from insectivorous bat, Taphozous melanopogon
| Autor: | Sahayanathan Gracy Jenifer, Hanumanthan Raghuram, Ganapathy Marimuthu |
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| Rok vydání: | 2021 |
| Předmět: |
DNA
Bacterial Escherichia Chitin Applied Microbiology and Biotechnology Microbiology Agar plate chemistry.chemical_compound Chiroptera RNA Ribosomal 16S Animals Phylogeny biology Hydrolysis Chitinases Escherichia fergusonii General Medicine 16S ribosomal RNA biology.organism_classification Gastrointestinal Microbiome chemistry Chitinase Taphozous melanopogon Nucleic acid biology.protein Bacteria |
| Zdroj: | Journal of basic microbiologyREFERENCES. 61(10) |
| ISSN: | 1521-4028 |
| Popis: | Chitinases are capable of hydrolyzing insoluble chitin into its oligo and monomeric parts and have received increased consideration because of their wide scope of biotechnological applications. The commercial application of microbial chitinase is appealing due to the relative ease of enormous production and to meet the current world demands. This study aimed at isolation and characterization of chitin degrading bacteria from the gut of Indian tropical insectivorous black-bearded tomb bat, Taphozous melanopogon. The isolated bacterial strains were characterized through biochemical analysis and nucleic acid-based approaches by 16S ribosomal RNA amplification and sequencing. The BLAST (Basic Local Alignment Search Tool) and phylogenetic analysis showed that the bacterial strain exhibited a close resemblance with Escherichia fergusonii. The chitinolytic activity of the E. fergusonii AMC01 was identified using supplemented colloidal chitin with agar medium. Compiling all, these findings would facilitate in constructing a database and presumably promote the use of E. fergusonii AMC01 as an efficient strain for the chitinase production. |
| Databáze: | OpenAIRE |
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