Endocannabinoids, through opioids and prostaglandins, contribute to fever induced by key pyrogenic mediators

Autor: Aleksander Roberto Zampronio, Carlos Amílcar Parada, Cristiane I. Zanoni, Glória Emília Petto de Souza, Daniel Fraga, Giles A. Rae
Rok vydání: 2015
Předmět:
0301 basic medicine
AM251
Male
medicine.medical_specialty
Cannabinoid receptor
Fever
Corticotropin-Releasing Hormone
Polyunsaturated Alkamides
Narcotic Antagonists
Immunology
Interleukin-1beta
Prostaglandin
(+)-Naloxone
Arachidonic Acids
Body Temperature
03 medical and health sciences
Behavioral Neuroscience
chemistry.chemical_compound
0302 clinical medicine
Piperidines
Receptor
Cannabinoid
CB1
Internal medicine
Medicine
Animals
Rats
Wistar
Endogenous opioid
Endothelin-1
Endocrine and Autonomic Systems
business.industry
Interleukin-6
Naloxone
Tumor Necrosis Factor-alpha
beta-Endorphin
Anandamide
Rats
030104 developmental biology
Endocrinology
chemistry
Systemic administration
ENDORFINA
Prostaglandins
Cytokines
Pyrazoles
lipids (amino acids
peptides
and proteins)
Endothelin receptor
business
030217 neurology & neurosurgery
medicine.drug
Endocannabinoids
Zdroj: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
ISSN: 1090-2139
Popis: This study aims to explore the contribution of endocannabinoids on the cascade of mediators involved in LPS-induced fever and to verify the participation of prostaglandins and endogenous opioids in fever induced by anandamide (AEA). Body temperature (Tc) of male Wistar rats was recorded over 6h, using a thermistor probe. Cerebrospinal fluid concentration of PGE2 and β-endorphin were measured by ELISA after the administration of AEA. Intracerebroventricular administration of the CB1 receptor antagonist AM251 (5μg, i.c.v.), reduced the fever induced by IL-1β (3ng, i.c.v.), TNF-α (250ng, i.c.v.), IL-6 (300ng, i.c.v.), corticotrophin release factor (CRH; 2.5μg, i.c.v.) and endothelin (ET)-1 (1pmol, i.c.v.), but not the fever induced by PGE2 (250ng, i.c.v.) or PGF2α (250ng, i.c.v.). Systemic administration of indomethacin (2mgkg(-1), i.p.) or celecoxib (5mgkg(-1), p.o.) reduced the fever induced by AEA (1μg, i.c.v.), while naloxone (1mgkg(-1), s.c.) abolished it. The increases of PGE2 and β-endorphin concentration in the CSF induced by AEA were abolished by the pretreatment of rats with AM251. These results suggest that endocannabinoids are intrinsically involved in the pyretic activity of cytokines (IL-1β, TNF-α, IL-6), CRH and ET-1 but not the PGE2 or PGF2α induced fevers. However, anandamide via CB1 receptor activation induces fever that is dependent on the synthesis of prostaglandin and opioids.
Databáze: OpenAIRE
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