Horseradish peroxidase functionalized gold nanorods as a label for sensitive electrochemical detection of alpha-fetoprotein antigen
| Autor: | Zilin Guo, Junqing Zhao, Xiaowei Han, Jinjin Guo, Yuzhong Zhang, Junchun Wang |
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| Rok vydání: | 2015 |
| Předmět: |
Biophysics
Analytical chemistry Carbon nanotube Electrochemistry Biochemistry Horseradish peroxidase law.invention Limit of Detection law medicine Humans Molecular Biology Horseradish Peroxidase Immunoassay Detection limit Nanotubes medicine.diagnostic_test biology Nanotubes Carbon Chemistry Benzidines Electrochemical Techniques Hydrogen Peroxide Cell Biology Enzymes Immobilized Primary and secondary antibodies humanities biology.protein Nanorod Gold alpha-Fetoproteins Differential pulse voltammetry Antibodies Immobilized Nuclear chemistry |
| Zdroj: | Analytical Biochemistry. 491:58-64 |
| ISSN: | 0003-2697 |
| Popis: | In this study, a novel tracer, horseradish peroxidase (HRP) functionalized gold nanorods (Au NRs) nanocomposites (HRP-Au NRs), was designed to label the signal antibodies for sensitive electrochemical measurement of alpha-fetoprotein (AFP). The preparation of HRP-Au NRs nanocomposites and the labeling of secondary antibody (Ab2) were performed by one-pot assembly of HRP and Ab2 on the surface of Au NRs. The immunosensor was fabricated by assembling carbon nanotubes (CNTs), Au NRs, and capture antibodies (Ab1) on the glassy carbon electrode. In the presence of AFP antigen, the labels were captured on the surface of the Au NRs/CNTs via specific recognition of antigen-antibody, resulting in the signal intensity being clearly increased. Differential pulse voltammetry (DPV) was employed to record the response signal of the immunosensor in phosphate-buffered saline (PBS) containing hydrogen peroxide (H2O2) and 3,3',5,5'-tetramethylbenzidine (TMB). Under optimal conditions, the signal intensity was linearly related to the concentration of AFP in the range of 0.1-100 ng ml(-1), and the limit of detection was 30 pg ml(-1) (at signal/noise [S/N] = 3). Furthermore, the immunoassay method was evaluated using human serum samples, and the recovery obtained was within 99.0 and 102.7%, indicating that the immunosensor has potential clinical applications. |
| Databáze: | OpenAIRE |
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